摘要
研究建立了麻痹性贝毒（PSP）毒素13种代表性化合物GTX14、dcGTX2—3、GTX5、C1—2、STX、NEO、dcSTX和dcNEO的高效液相色谱-串联质谱（HPLC-MS／MS）检测方法。以0．1mol／I。盐酸溶液对贝类组织中的PSP毒素进行提取，再以C18固相萃取柱对提取液进行净化并超滤离心，最后以高效液相色谱-串联质谱测定。PSP毒素13种代表性化合物在各自浓度范围内线性良好，回收率为71．2％～97．5％，精密度为3．6％～16．3％。检测低限范围为5．2～13．4ug／kg。该方法操作简便、灵敏度强，重现性好，准确性高，可以满足日常对于PSP毒素的检测要求。
Researching and building the HPI.C-MS/MS method of determinating thirteen PSP typical compounds GTX1-4, dcGTX2-3, GTXS, C1-2, STX, NEO, dcSTX and dcNEO. The PSP toxins in shellfish were extracted by 0. 1 mol/L hydrochloric acid aqueous solution, and purified by C18 solid phase extraction column, then ultrafiltrated, determinated by HPI.C-MS/MS at last. The thirteen PSP typical compounds have good linear relation in each concentration range, the recovery is in the range of 71.2 %-97.5 %0, the precision is in the range of 3.6 %-16.3 %. The LOQisin the range of 5.2- 13.4μg/kg. The method can be easy to operate, and has good sensitivity, reproducibility and accuracy. The method can satisfy the detection requirement of PSP toxins.
出处
《海洋湖沼通报》
CSCD
北大核心
2013年第4期66-72,共7页
Transactions of Oceanology and Limnology
基金
国家质检总局科技项目<贝类毒素标准物质的研制及应用研究>2010IK158资助
