转新疆野生樱桃李psoRPM1基因烟草抵抗南方根结线虫

psoRPM1 Gene from Prunus sogdiana Indicated Resistance to Root-knot Nematode in Tobacco

从新疆野生樱桃李(Prunus sogdiana)抗南方根结线虫单株中分离克隆得到一个能响应南方根结线虫侵染的抗病基因psoRPM1,利用农杆菌介导方法将其导入烟草中,并对其抗性进行了鉴定。结果显示,构建的pCAMBA1305.1表达载体能成功地将psoRPM1基因导入高度感病的烟草‘W38’中,并获得了9株转基因单株。对转基因单株进行抗性鉴定,结果显示转入psoRPM1基因后使烟草‘W38’对南方根结线虫的抗性由高度感病变为中度抗病。这一结果预示着psoRPM1基因可能是一个新的与抗根结线虫相关的基因。

The root-knot nematode, especially Meloidogyne incongnita is one of the most harmful pests could induce many crop diseases. Xinjiang wild cherry plum （Prunus sogdiana）is a kind of wild and endemic specie in China with significant resistant effect against M. incognita. Here we isolated a gene from Xinjiang wild cherry plum, which named psoRPM1, having responses to the infection of M. incognitain the resistant plant. Using Agrobacterium-mediated transgenic technology, psoRPMI gene was introduced into tobacco varieties ＇W38＇, which is highly susceptible to M. incognita. Then the transgenic tobaccos and plants of control group were inoculated with M. incognita. After growing for 60 d, the psoRPM1-W38 showed varying degrees of resistance to M. incognita, even some of the plants nearly had no root-knots. Comparing to the tobaccos of the control group, it is suggested t^at psoRPM1 gene is capable of helping the tobacco ＇W38＇ set up resistance mechanism against the infection of M. incognita and finally predict the psoRPM1 is a new root-knot nematode resistance gene＇candidate.