TG2在胃癌组织中表达的临床意义

Clinical significance of expression of tissue transglutaminase 2 in gastric carcinoma

目的:了解组织型转谷氨酰胺酶2(tissue transglutaminase 2,TG2)在胃中的表达情况及其临床意义,为寻找早期诊断胃癌相关的分子标志物提供实验依据.方法:采用RT-PCR和Western blot检测TG2在胃癌组织中的表达;应用免疫组织化学染色检测TG2在胃癌组织中的表达,并分析其在胃癌组织中表达的临床病理学意义.结果:(1)RT-PCR和Western blot结果均显示,TG2在正常胃黏膜和胃癌组织中的表达强度分别为0.274±0.051 vs 0.671±0.105和0.317±0.032 vs 0.918±0.117,与正常胃黏膜组织比较,TG2在胃癌组织中的表达明显增加(P<0.05);(2)免疫组织化学染色结果显示,TG2蛋白在正常胃黏膜和胃癌组织中的阳性表达率分别为33.33%(8/24)和71.74%(33/46),胃癌组织中的阳性表达率明显高于正常胃黏膜组织(P<0.05).结论:TG2蛋白高表达与胃癌的发生密切相关.

AIM： To assess the clinical significance of expres- sion of tissue transglutaminase 2 （TG2） in gastric carcinoma, so as to provide a new clue for finding new specific markers of gastric cancer. METHODS： The expression of TG2 in gastric cancer tissues was detected by RT-PCR, Western blot and immunohistochemistry. The clinical significance of TG2 expression in gastric cancer was analyzed. RESULTS： The result of RT-PCR and Western blot analyses showed that the mRNA and pro- tein expression levels of TG2 were significantly lower in normal gastric mucosa than in gastric cancer tissues （0.274 ± 0.051 vs 0.671 ± 0.105, 0.317 ±0.032 vs 0.918 ± 0.117, both P 〈 0.05）. The im- munohistochemical results demonstrated that the positive expression rate of TG2 protein in normal gastric mucosa was significantly lower than that in gastric cancer tissues [33.33% （8/24） vs 71.74% （33/46）, P 〈 0.05]. CONCLUSION： Overexpression of TG2 protein may be closely related to the occurrence of gas- tric cancer.