星形细胞瘤MRI扩散加权成像、肿瘤细胞密度与缺氧诱导因子-1α表达水平的相关性

Correlation Between Diffusion Weighted Imaging, Tumor Cellularity and Expression Level of Hypoxia-inducible Factor-1α in Cerebral Astrocytoma

目的探讨高级别和低级别星形细胞瘤MRI DWI、肿瘤细胞密度与缺氧诱导因子-1α(HIF-1α)表达水平的相关性。资料与方法对术后病理确诊的33例星形细胞瘤患者术前行MRI DWI检查,并计算肿瘤实质部分的ADC值;用Scion Image 4.0.3.2软件计算肿瘤细胞密度;采用免疫组化检测HIF-1α的表达情况。结果低级别星形细胞瘤的平均ADC值高于高级别星形细胞瘤,差异有统计学意义(t=7.300,P<0.001)。高级别星形细胞瘤平均细胞密度值明显大于低级别星形细胞瘤,差异有统计学意义(t=-3.845,P<0.01)。低级别和高级别星形细胞瘤中均有HIF-1α表达,其表达强度分别为(20.08±10.01)%、(47.91±19.03)%。肿瘤ADC值与HIF-1α标记指数、肿瘤细胞密度值呈显著负相关(r=-0.756、-0.617,P<0.001),HIF-1α和肿瘤细胞密度值呈显著正相关(r=0.622,P<0.001)。结论 ADC值有助于初步判定星形细胞瘤的性质,对低级别和高级别星形细胞瘤有一定的鉴别价值。HIF-1α在星形细胞瘤中的作用应扩大样本量进一步深入研究。

Purpose To evaluate the correlation among diffusion weighted imaging （DWI）, tumor Cellularity and hypoxia-inducible factor-lα （HIF-1α） for the high and low grade astrocytoma. Materials and Methods DWI was applied with 33 patients with astrocytoma confirmed by pathology, and ADC value was measured. Tumor Cellularity was measured using Scion Image 4.0.3.2. The expression of HIF-lα was tested using immunohistochemisty. Results Mean ADC value was higher in the low grade astrocytoma than that in the high grade astrocytoma （t=7.300, P〈0.001）. The tumor Cellularity was higher in the high grade astrocytoma than that in the low grade astrocytoma （t= -3.845, P〈0.01）. HIF- 1 α expression could be demonstrated in the low grade [（20.08 ± 10.01 ）%] and high grade [（47.91 ± 19.03）%] astrocytoma. The negative correlation was demonstrated between ADC value and HIF-1α and tumor Cellularity （r=- 0.756, -0.617; P〈0.001）. The positive correlation was demonstrated between H IF-lα and tumor Cellularity （t=0.622, P〈0.001）. Conclusion ADC value can be used to discriminate the low and high grade astrocytoma, and the role of HIF-lα should be further to study with enlarged sample.