摘要
目的:探讨多囊卵巢综合征(PCOS)患者血清蛋白因子的变化和临床意义。方法:采用抗体蛋白质芯片检测PCOS患者与正常对照女性血清,筛选174种蛋白因子,根据结果选择2种既往无报道的因子进行ELISA验证,并进行统计分析。结果:有23种因子在PCOS组升高,包括生长因子类7种和炎症反应因子类6种,其余10种分别为血管生长素(angiogenin)、巨噬细胞炎症蛋白1δ(MIP-1-delta)、肺活化调节趋化因子(PARC)、脂联素(Acrp30)、金属蛋白酶组织抑制物2(TIMP-2)、TNF相关凋亡诱导配体受体3(TRAIL-R3)、E-选择素(E-selectin)、潜在相关肽(LAP)、L-选择素(L-selectin)和泌乳素(prolactin)。174种因子中PCOS组表达强度增高达3倍以上的有白细胞介素1受体II(IL-1RII)、prolactin、IL-7、胰岛素样生长因子结合蛋白1(IGFBP-1)、IGFBP-4、IL-5受体α(IL-5Ra)、血管内皮生长因子受体2(VEGFR2)、细胞间粘附分子1(ICAM-1)和MIP-1-delta。3种因子在PCOS组降低,分别为生长调节癌基因(GRO)、IL-8和Siglec-5。选择血小板源性生长因子BB(PDGF-BB)及Acrp30进行ELISA验证,结果显示差异有统计学意义(P<0.05)。结论:PCOS患者多种血清蛋白因子发生改变,它们之间的相互作用可能是其发病原因之一。
AIM: To study the changes and clinical significance of serum protein factors in the patients with polycystic ovary syndrome (PCOS). METHODS: The relative expression levels of 174 ser protein factors in 5 PCOS pa- tients and 5 control women were assayed simultaneously by Antibody Protein Array 2000,in which 2 factors were chosen to test and reconfirm the results by ELISA in 30 cases of PCOS patients and 30 cases of controls. RESULTS: Among 174 serum protein factors, the factors that elevated in PCOS patients were angiogenin, EGF, IGFBP-1, IGFBP-4, IL-7, MIP- 1-delta, PARC, PDGF-BB, Acrp30, EGFR, ICAM-1, sTNF-RI, TIMP-2, TRAIL-R3, E-selectin, IL-1RII, IL-SRa, LAP, L-selectin, M-CSFR, PDGF-AA, prolactin and VEGFR2, while the factors that decreased in PCOS patients were GRO, IL-8 and Siglec-5. Nine factors were increased 3 times more than the controls(IL-1R-II, prolactin, IL-7, IGFBP-1, IL-SRa, IGFBP-4, VEGFR2, ICAM-1 and MIP-l-delta). PDGF-BB and Acrp30 were tested by ELISA in 60 samples and there were significant differences between the 2 groups. CONCLUSION : The changes of the protein factors in serum might play an important role in the pathogenesis of PCOS.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第12期2251-2255,共5页
Chinese Journal of Pathophysiology
基金
江苏省六大高峰人才项目(No.2012-WS-098)