多囊卵巢综合征患者血清中蛋白因子的变化及其临床意义

Changes and clinical significance of serum protein factors in patients with polycystic ovary syndrome

目的:探讨多囊卵巢综合征(PCOS)患者血清蛋白因子的变化和临床意义。方法:采用抗体蛋白质芯片检测PCOS患者与正常对照女性血清,筛选174种蛋白因子,根据结果选择2种既往无报道的因子进行ELISA验证,并进行统计分析。结果:有23种因子在PCOS组升高,包括生长因子类7种和炎症反应因子类6种,其余10种分别为血管生长素(angiogenin)、巨噬细胞炎症蛋白1δ(MIP-1-delta)、肺活化调节趋化因子(PARC)、脂联素(Acrp30)、金属蛋白酶组织抑制物2(TIMP-2)、TNF相关凋亡诱导配体受体3(TRAIL-R3)、E-选择素(E-selectin)、潜在相关肽(LAP)、L-选择素(L-selectin)和泌乳素(prolactin)。174种因子中PCOS组表达强度增高达3倍以上的有白细胞介素1受体II(IL-1RII)、prolactin、IL-7、胰岛素样生长因子结合蛋白1(IGFBP-1)、IGFBP-4、IL-5受体α(IL-5Ra)、血管内皮生长因子受体2(VEGFR2)、细胞间粘附分子1(ICAM-1)和MIP-1-delta。3种因子在PCOS组降低,分别为生长调节癌基因(GRO)、IL-8和Siglec-5。选择血小板源性生长因子BB(PDGF-BB)及Acrp30进行ELISA验证,结果显示差异有统计学意义(P<0.05)。结论:PCOS患者多种血清蛋白因子发生改变,它们之间的相互作用可能是其发病原因之一。

AIM： To study the changes and clinical significance of serum protein factors in the patients with polycystic ovary syndrome （PCOS）. METHODS： The relative expression levels of 174 ser protein factors in 5 PCOS pa- tients and 5 control women were assayed simultaneously by Antibody Protein Array 2000,in which 2 factors were chosen to test and reconfirm the results by ELISA in 30 cases of PCOS patients and 30 cases of controls. RESULTS： Among 174 serum protein factors, the factors that elevated in PCOS patients were angiogenin, EGF, IGFBP-1, IGFBP-4, IL-7, MIP- 1-delta, PARC, PDGF-BB, Acrp30, EGFR, ICAM-1, sTNF-RI, TIMP-2, TRAIL-R3, E-selectin, IL-1RII, IL-SRa, LAP, L-selectin, M-CSFR, PDGF-AA, prolactin and VEGFR2, while the factors that decreased in PCOS patients were GRO, IL-8 and Siglec-5. Nine factors were increased 3 times more than the controls（IL-1R-II, prolactin, IL-7, IGFBP-1, IL-SRa, IGFBP-4, VEGFR2, ICAM-1 and MIP-l-delta）. PDGF-BB and Acrp30 were tested by ELISA in 60 samples and there were significant differences between the 2 groups. CONCLUSION ： The changes of the protein factors in serum might play an important role in the pathogenesis of PCOS.