摘要
目的:观察葛根素治疗糖尿病小鼠肾间质纤维化(renal interstitial fibrosis,RIF)过程中肾小管上皮-间质转化(epithelial-mesenchymal transition,EMT)相关蛋白表达的变化,为临床药物治疗糖尿病RIF提供实验依据。方法:将16只2型糖尿病模型KKAy小鼠随机分为模型组(n=8)和葛根素注射液治疗组(n=8)。8只C57BL/6J小鼠作为正常组。用药组小鼠14周龄开始以腹腔注射的方式给药,观测小鼠日常状态及血糖变化,于24周龄处死小鼠,取肾脏分别观察肾组织病理形态学变化,免疫组织化学法检测小鼠肾组织中α-平滑肌肌动蛋白(alpha-smooth muscle actin,α-SMA)、转化生长因子β1(transforming growth factorβ1,TGF-β1)和转化生长因子βI型受体(TGF-β-RI)蛋白的表达。结果:形态学观察可见模型组发生明显的纤维化改变,而经葛根素注射液治疗后,小鼠肾间质基质轻度增多,病变较轻微。治疗组KKAy小鼠肾组织中较模型组小鼠肾组织中α-SMA、TGF-β1和TGF-β-RI蛋白的表达减少。结论:葛根素注射液可在一定程度上减少α-SMA的表达,抑制KKAy小鼠肾脏组织中TGF-β1和TGF-β-RI蛋白表达,阻断并逆转EMT的过程,延缓肾间质纤维化的发生和发展。
AIM: To observe the effect of puerarin injection on the expression of epithelial-mesenchymal tran- sition-related proteins in KKAy mice with renal interstitial fibrosis (RIF). METHODS: Sixteen KKAy mice were randomly divided into model group (n = 8) and puerarin injection treatment group (n = 8 ), and 8 C57BL/6J mice were used as nor- real controls. The mice in treatment group were intraperitoneally given puerarin injection from the 14th week. The blood glucose levels were observed on a daily basis. The mice were sacrificed at the 24th week. The renal pathological changes were observed under light microscope. The expression of a-smooth muscle actin (ot-SMA), transforming growth factor ~t (TGF-~,) and transforming growth factor ~ type I receptor (TGF-^-RI) in the renal tissues were examined by immunohis- tochemical staining. RESULTS : Fibrosis was found in the KKAy mice of model group, while the mice in treatment group showed a slight increase in renal interstitium. Treatment with puerarin injection decreased the protein expression levels of α-SMA, TGF-β1 and TGF-β-RI in the kidney tissues as compared with those in model group. CONCLUSION: Puerarin injection reduces the expression of α-SMA, and restrains the protein expression of TGF-β1 and TGF-β-RI in the kidney tis- sue of KKAy mice. These changes may inhibit and reverse the process of epithelial-mesenchymal transition, thus delaying the occurrence and development of RIF.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第12期2263-2267,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81072926)
北京中医药大学创新团队项目(No.2011-CXD-04)
关键词
肾间质纤维化
上皮-间质转化
葛根素注射液
转化生长因子β
I型受体
Α-平滑肌肌动蛋白
Renal interstitial fibrosis
Epithelial-mesenchymal transition
Puerarin injection
Transforming growth factor beta type I receptor
Alpha-smooth muscle actin