摘要
应用双链RNA(dsRNA)诱导的RNA干扰(RNAi)技术原理,将体外合成的源于白背飞虱肌动蛋白(Actin)基因的dsRNA通过膜饲喂法饲喂其2龄若虫.通过优化人工饲料、dsRNA浓度和饲喂时间,成功地干扰了白背飞虱Actin基因的表达,并引发白背飞虱的死亡,从而建立了白背飞虱体内的RNAi体系.
The potential of RNA interference （RNAi） induced by double-strand RNA （dsRNA） was tested in white-backed plan- thopper （WBPH）. dsRNAs corresponding to the Actin gene of WBPH were produced in vitro and applied to the 2nd instar nymphs of WBPH through a membrane-feeding method. After optimizing the artificial diet, dsRNA concentration and feeding time, the ex- pression of Actin in cells of WBPH was significantly reduced, leading to death of the insects prematurely. This indicated that the in vivo RNAI system for WBPHs was established.
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2013年第6期579-583,共5页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金重点项目(3113044)
福建省杰出青年科学基金项目(2011J06008)
教育部高等学校博士学科点专项科研基金(20113515130002)
863计划(2012AA101505)
关键词
白背飞虱
RNA干扰
ACTIN基因
双链RNA
膜饲喂法
whlte-backed planthopper
RNA interference （RNAi）
Actin gene
double-stranded RNA （dsRNA）
membrane-feeding
