血清学方法和实时荧光定量PCR方法在新型布尼亚病毒检测中的应用研究

Comparison of serological methods and real time thrombocytopenia syndrome Bunyavirus

目的探讨血清学检测方法和实时荧光定量PCR方法在新型布尼亚病毒检测中的应用。方法连续采集烟台地区莱州、蓬莱疑似发热伴血小板减少综合征患者血清，分别用胶体金实验方法、ELISA实验方法检测抗新型布尼亚病毒IgM抗体，实时荧光定量PCR方法检测新型布尼亚病毒核酸，SPSS18．0统计结果。结果胶体金方法和ELISA方法检测结果总符合率为96．67％，一致性KAPPA检验K系数为0．933；胶体金方法和实时荧光定量PCR方法检测结果均受病程影响（两者P值均小于0．05）；实时荧光定量PCR2疗法检测不受年龄、性别影响（P均〉0．05）。结论新型布尼亚病毒检测发病12天以前推荐使用实时荧光定量PCR方法，发病16天以后，可使用血清学方法检测抗新型布尼亚病毒IgM抗体。

Objective To compare serological methods with real time RT-PCR in detection of severe fever with thrombocytopenia syndrome Bunyavirus. Method Serum of severe fever with thrombocytopenia syndrome was collected in Penglai and Laizhou in Yantai. Anti-severe fever with thrombocytopenia syndrome Bunyavirus （SFTSV） IgM was detected using gold colloid and ELISA, nucleic acid of severe fever with thrombocytopenia syndrome Bunyavirus was detected by real-time PCR. Statistics of the results were done using SPSS 18.0. Result The coincidence of ELISA and gold colloid was 96.97% and the kcoefficient was 0. 933 ; the course of disease influences the results notablely both gold colloid and real-time PCR（P 〈 0. 05 ） and real-time PCR was not influenced by patients age or sex （ P 〉 0. 05 ）. Conclusion When the course of disease less than 12 days, real-time PCR was recommended and when the course of disease more than 16 days, serological methods can use to detect the anti-SFTSV IgM.