摘要
卵黄蛋白原(Vitellogenin,Vtg)是检测环境雌激素的重要生物标志物,雄性鱼类Vtg mRNA是检测环境雌激素的新兴生物标志物。为研究BaP是否具有环境雌激素效应,本试验对罗非鱼Oreochromis niloticus Vtg mRNA进行研究,用总RNA提取试剂盒提取得到雌鱼肝脏总RNA,以转录得到的cDNA为模板,以Primer设计得到的引物,得到422 bp的罗非鱼卵黄蛋白原特异性cDNA扩增片段;采用荧光定量PCR法检测10、50μg·L-1的BaP暴露对罗非鱼肝脏Vtg基因表达的影响,结果发现4 d、17 d时雄性罗非鱼肝脏内Vtg mRNA表达量与对照组无差异(P>0.05),10 d时10、50μg·L-1 2个剂量组Vtg mRNA表达量显著高于对照组(P<0.01),分别是对照组的113.18倍和121.79倍。结果意味着BaP可能具有环境雌激素效应。
Vitellogenin （Vtg） is an important biomarker for monitoring environmental estrogens （EEs） in aquatic environment. Vtg mRNA in male fish is a new kind of biomarker. In order to research whether BaP has environmental estrogen effect, the Vtg mRNA was also studied in this paper. Total liver RNA was extracted from the liver by Trizol kit. The RT-PCR was conducted with vitellogenin special primers designed by Esterhuyse and by ourselves. The isolated 422 bp tilapia vitellogenin eDNA were obtained. The effect of BaP on tilapia liver Vtg mRNA expression was detected with the help of real-time PCR. Results showed that there were no significant differences ofVtg gene expression among control group and the exposure concentrations of 10 ~tg.L1 and 50 Bg.L-1 in day 4 and 17（P〉0.05）, in day 10, the Vtg mRNA level increased by 113.18% and 121.79% respectively in 10 ~tg-Ll group and 50 ~tg＇L-1 group which were significantly higher than that in control group （P〈0.01）.
出处
《生态环境学报》
CSCD
北大核心
2013年第11期1837-1840,共4页
Ecology and Environmental Sciences
基金
国家现代农业产业技术体系项目(CARS-49)
中央级公益性科研院所基本科研业务费专项资金(2011JBFA16)
