MiR-182在结直肠癌中的表达及其对结直肠癌细胞迁移能力的影响

Expression of miR-182 in colorectal cancer and its effect on the migration of colorectal cancer cells

目的检测miR-182在结直肠癌中的表达情况及与临床病理参数的关系,探讨其对结直肠癌细胞迁移能力的影响。方法实时荧光定量PCR(qRT-PCR)检测86例患者结直肠癌与对应癌旁组织中miR-182的表达情况,分析其与结直肠癌临床病理参数之间的关系。体外用miR-182 mimics和阴性对照转染结直肠癌HT-29细胞,将实验分为miR-182组、阴性对照组和空白组,transwell小室实验观察miR-182对HT-29细胞迁移能力的影响。结果 MiR-182在结直肠癌的表达显著高于癌旁(P<0.001),其表达水平与患者的浸润程度(P=0.028)、淋巴结转移(P=0.003)、远处转移(P=0.006)和临床分期(P=0.005)密切相关,而与年龄、性别、肿瘤类型、肿瘤大小和分化程度无相关性(P均>0.05)。体外实验进一步表明,miR-182过表达的HT-29细胞迁移数显著高于阴性对照组和空白组(P均<0.05)。结论 MiR-182在结直肠癌中高表达且与肿瘤的转移及进展有关,体外能增强结直肠癌细胞的迁移能力,提示miR-182可作为预防和治疗结直肠癌转移的潜在靶点。

Objective To detect the expression level of miR-182 in colorectal cancer, explore the correlations between miR-182 expression and clinicopathological features, and investigate its effect on the migration of colorectal cancer ceils in vitro. Methods Quantitative real-time PCR （qRT-PCR） was used to measure the expression of miR-182 in 86 pairs of colorectal cancer and corresponding adjacent noncancerous tissues, and the correlations between miR-182 expression and clinicopathological features were further analyzed. HT-29 cells were transiently transfected with miR-182 mimics and miR-negative control, and the experiment was divided into miR-182 over-expression group, negative control group and blank group. In addition, the migration ability was detected by transwell assay in vitro. Results The expression of miR-182 was significantly higher in colorectal cancer tissues compared with corresponding adjacent noncancerous tissues （ P 〈0.001 ）. Its level was significantly correlated with local invasion （ P = 0. 028 ）, lymph nodes metastasis （ P = 0. 003 ）, distant metastasis （ P = 0.006 ）, and clinical stage （ P = 0. 005 ）, respectively. However, no correlation was observed between miR-182 and age or other clinicopathological parameters （all P〉0.05）. The migration cells were significantly higher in miR-182 over-expression group than negative control group and blank group. Conclusion MiR-182 is up-regulated in colorectal cancer and that high miR-182 expression is associated with the metastasis and progression of colorectal cancer. In addition, the up-regulated miR-182 promotes colorectal cancer cell migration in vitro, suggesting a potential target for the prevention and therapy of metastasis in colorectal cancer.