原发性高血压伴肥胖患者血浆RAAS激素水平和CYP11B2-344C/T基因多态性的相关性

Correlation Between Plasma RAAS Hormone Levels and CYP11B2-344C /T Gene Polymorphism in Essential Hypertension Patients with Obesity

目的通过检测原发性高血压伴肥胖患者血浆肾素-血管紧张素·醛固酮系统（RAAS）激素水平和醛固酮合成酶CYP11B2～344c／T基因多态性，探讨原发性高血压伴肥胖患者CYP11B2—344C／T易惠基因型以及基因多态性与RAAS的关系。方法随机选取1～2级原发性高血压患者60例，其中高血压伴肥胖者30例，单纯高血压者30例；同期体检中心体检者60例，其中单纯肥胖者30例，健康者30例。采用PCR—RFLP和琼脂糖凝胶电泳等方法检测CYP11B2—344C／T基因多态性，用放射免疫法检测血浆RAAS水平。结果1Tr基因型例数与TC＋CC基因型例数进行多重比较，有显著差异（P〈0．05），其中以原发性高血压伴肥胖组差异最为显著；T与c等位基因例数进行多重比较，有显著差异（P〈0．05），其中以原发性高血压伴肥胖组差异最为明显。按基因型分组统计分析各组血浆肾素、血管紧张素Ⅱ及醛固酮水平，1-r基因型组显著高于TC、CC基因型组（P〈0．05）。结论原发性高血压伴肥胖患者CYP11B2—344c／T基因型以1Tr基因型为主，等位基因以T等位基因为主。Irr基因型血浆RAAS激素水平各组份比TC、CC基因型显著升高，TT基因型可能为原发性高血压伴肥胖患者易感基因型。

Aim To study renin-angiotensin-aldosterone system （RAAS） hormone levels and CYPllB2 -344C/T gene polymorphism in essential hypertension patients with obesity, discuss genetic susceptibility of CYP11 B2 -344C/T genotype and the correlation between susceptible gene and RAAS levels. Methods 60 cases of patiens with 1 ~ 2 grade essential hypertension （30 cases essential hypertension patients with obesity, 30 cases essential hyperten- sion patients without obesity）, and 60 people from the Examination Center （30 cases obesity people without hypertension, 30 cases healthy people） were enrolled randomly. CYP11 B2 -344C/T gene polymorphisms were analyzed using polymer- ase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis methods, plasma RAAS concen- tration were detected by radioimmunoassay. Results Multi-compared with number of cases of TT and TC ＋ CC geno- type, there were significant differences （ P 〈 0. 05 ）. And differences in essential hypertension with obesity group was the most significant; multi-compared with the number of T and C alleles, significant differences were found （ P 〈 0.05 ）, and differences in essential hypertension with obesity group was predominant. Plasma renin, angiotensin II , aldosterone con- centrations in genotype TY cases were much higher than those of TC and CC genotype （ P 〈 0. 05 ）, but there was no signifi-cant differences between CC and TC genotype cases （ P 〉 0. 05 ）. Conclusions In CYP11 B2 - 344C/T gene polymor- phism, TT genotype may be susceptible genotype of essential hypertension patients without obesity, and T allele may be susceptible one of them. Each component of plasma RAAS hormone levels in the TT genotype cases was significantly higher than the CC and the TC genotype ones.