5-羟色胺1Dβ受体基因861G/C多态性与强迫症关联的病例对照研究

A case-control study of serotonin 1Dβ receptor gene 861G /C polymorphism in obsessive-compulsive dis-order

目的：探讨5-羟色胺lDr3受体（5-HTRlDβ）基因861G／C多态性与强迫症的关联性。方法：对239例强迫症（强迫症组）患者和337名健康对照（对照组）通过聚合酶链式反应与限制性片段长度多态性基因分型技术对5-HTRlDB基因单核苷酸多态性位点861G／C进行基因分型。结果：861G／C位点基因型频率分布两组问比较差异有统计学意义（X2=7．59，df=2，P=0．023），而等位基因频率分布差异无统计学意义；杂合子GC基因型与纯合子（GG＋CC）基因型（X。=4．59，P=0．03，OR=1．44，95％CI：1．03～2．01）或CC基因型与GG＋GC基因型（X2=6．85，P=0．009，OR：0．58，95％C1=0．38～0．87）两组间比较差异有统计学意义，而GG基因型与GC＋CC基因型差异无统计学意义。两组女性之间比较，基因型（）f。=11．98，df=2，P：0．0025）与等位基L天J频率（X。=4．90，af=1，P=0．03，OR=1．51，95％C1=1．05～2．17）分布差异有统计学意义，而两组男性之间比较，基因型与等位基因频率分布差异无统计学意义。，强迫症晚发（〉16岁）组与对照组基因型频率分布差异有统计学意义（×。=6．45，妙=2，P=0．04），而等位基因频率分布差异无统计学意义；强迫症早发（≤16岁）组、强迫症临床3个亚组基因型与等位基因频率分布上与对照组之间差异均无统计学意义。结论：5-HTR1Dβ861G／C多态性可能与强迫症和晚发型强迫症仔在关联；G等位基因可能是女性强迫症的风险因子。

Objective： To investigate the relevance between serotonin 1D[3 receptor （ 5-HTR1 D[3） gene 861G/C polymorphism and obsessive-compulsive disorder （ OCD ）. Method ： Genotyping for 5-HTR1DI3 gene 861G/C was perfomled for 239 OCD patients and 337 health control by the polymerase chain reaction and re- striction fragment length polymorphism techniques. Results：There was statistically significant difference in genotypic frequency distritmtions （X2 = 7.59, df = 2, P = 0. 023 ） , but there was no statistically significant differ- ence in allelic frequency distributions between OCD patients and health controls;there were statistically signifi- cant differences in heterozygote GC genotype vs homozygote （GG ＋ CC） genotype （X2 = 4.59 ,P = 0. 03, OR = 1.44,95% CI = 1.03-2.01 ） or CC genotype vs GG ＋ GC genotype （ X2 = 6.85, P = 0. 009, OR = 0. 58,95% CI = 0.38-0.87 ）, however no statistically significant differences were found in GG genotype vs GC ＋ CC geno- type between OCD patients and health controls. The frequency distribution of genotype （ X2 = 11.98, df = 2, P = 0. 0025 ） and allele （ X2 = 4.90, df = 1 ,P = 0, 03, OR = 1.51,95 % C1 = 1.05-2.17 ） had statistically signifi- cant differences in OCD female pa6ents compared to female controls ,whereas there were no statistically signifi- cant differences in OCD male patients compared to male controls;there were statistically significant differences in the frequency distribution of genotype （X2 = 6.45 ,dr= 2 ,P = 0.04 ） , whereas there were no statistically sig- nificant differences in that of allele（ P 〉 0.05 ） between the late-onset OCD patients and health controls, com- pared to control, the frequency distribution of genotype and allele had no statistically significant differences in early-onset OCD patients and the same results to 3 clinical subgroups genotypes of OCD. Conclusion ： 5- HTRI D[3861G/C gene polymorphism is probably relevant to OCD and late-onset OCD while G allele may be the risk factor of female OCD patients.