不同剂量链脲佐菌素建立妊娠糖尿病小鼠模型稳定性比较

Comparison on the stability of the mouse model of gestational diabetes established by different doses of streptozotocin

目的探讨链脲佐菌素方法建立妊娠糖尿病小鼠模型中不同给药剂量对成模率及稳定性的影响。方法将40只C57BL/6J孕鼠随机分为对照组(10只)和实验组(链脲佐菌素低、中、高剂量组,每组10只),对照组一次性腹腔注射等剂量的柠檬酸缓冲液,实验组分别予一次性腹腔注射现配链脲佐菌素溶液20 mg/kg、50 mg/kg、100 mg/kg,之后均给予普通饲料喂养,自由进水。分别于妊娠第3、10、18天测空腹血糖与体质量,并观察饮水量与尿量变化,比较孕鼠成模情况。结果 20 mg/kg STZ注射组成模率为40%,50 mg/kg链脲佐菌素组成模率为70%,100 mg/kg STZ注射组成模率为50%。空腹血糖与对照组相比较其高血糖状态持续时间长,且体质量明显下降,差异具有统计学意义(P<0.05)。结论链脲佐菌素50 mg/kg腹腔注射C57BL/6J孕鼠是建立妊娠糖尿病小鼠模型的最佳剂量,具有较好的稳定性。

Objective To investigate the effect of different doses of streptozotocin （STZ） on the stability of the mouse model of gestational diabetes established by streptozotocin. Methods Forty C57BL/6J pregnant mice were randomly divided into the control group （10 mice） and the streptozotocin group （20 mg/kg STZ group, 50 mg/kg STZ group and 100 mg/kg STZ group, each with l0 mice）. The mice were injected intraperitoneally with equal dose of ci^ate buffer, STZ solution 20 mg/kg, 50 mg/kg, 100 mg/kg, respectively, according to their assigned groups, and then they were given normal feeding. The fasting blood glucose and body mass were measured on the third, 10th, 18th day of pregnancy, and the water intake and urine output changes were also observed. Finally the modeling rates of the pregnant mice were compared between the different groups. Results The modeling rate was found to be 40% for 20 mg/kg STZ group, 70% for 50 mg/kg STZ group, 50% for 100 mg/kg STZ group. Compared with the control group, the levels of fasting blood glucose was significantly higher in 50 mg/kg STZ group, and the body weight was signifi- cantly lower （P〈O.05）. The state of hyperglycemia retained continually and steadily. Conclusion 50 mg/kg is the op- timal dose for intraperitoneal injection of streptozotocin into C57BL/6J pregnant mice to establish gestational diabetes mouse model, with good stability.