肾康丸对2型糖尿病大鼠肾脏TRAIL系统表达的影响

Effects of Shenkangwan on the expression of tumor necrosis factor-related apoptosis-inducing ligand system in type 2 diabetic rats

目的:观察肾康丸治疗对2型糖尿病(T2DM)大鼠肾组织TRAIL系统表达的影响,探讨其肾脏保护作用机制。方法:建立T2DM大鼠模型。成模后将大鼠随机分为2组:模型对照组(DM)、肾康丸组(DS);另设正常对照组(C)。分组干预,全自动生化仪检测高密度脂蛋白胆固醇(FBG)、总胆固醇(TC)及空腹血糖(FBG)(HDL-C);ELISA法检测尿微量白蛋白α1-MG(Uα1-MG)、24 h尿微量白蛋白(24h UmAlb),Q-RT-PCR法检测TRAIL、DR4、DcR2mRNA在肾组织表达,HE染色法观察大鼠肾组织病理变化。结果:随着实验时间的延长,DM组FBG及TC升高,HDL-C降低,而DS组FBG及TC降低,HDL-C升高;与C组相比,第4周、第8周DM组大鼠Uα1-MG、24h UmAlb均显著升高(P<0.01,P<0.01),肾组织DcR2 mRNA表达显著增强(P<0.01);而TRAIL、DR4 mRNA表达显著减弱(P<0.01),并且DM组大鼠出现了肾脏组织病理损害;DS组各指标均呈相反改变,肾脏病理损害减轻。结论:肾康丸通过影响TRAIL系统的表达,对T2DM大鼠具有显著的肾保护作用。

Aim：To explore the effect of Shenkangwan on the expression of tumor necrosis factor-relat-ed apoptosis-inducing ligand system（TRAIL）in the kidneys of type 2 diabetes rats.Methods：The rat models of type 2 diabetes were randomly divided into diabetic model group （DM）and Shenkangwan treat-ment group （DS）,with the rats fed with normal chow as the control group （C）.After treatment,The FBG,TC and HDL-C were detected by automatic biochemical analyzer.The levels of urine α1-MG （Uα1-MG）and 24 hour urine mAlb （24h UmAlb）were detected by ELISA,and the expressions of TRAIL,death receptor 4 （DR4 ）and decoy receptor 2 （DcR2 ）protein and mRNA in the kidney were measured by immune histochemistry and quantitative real-time PCR（Q-RT-PCR）,Then we investigated their renal pathological change under optical microscope with the coloration method of Hematoxylin and E-osin（HE）.Results：The levels of FBG and TC increased while HDL-C reduced in the DMgroup,these changes were all reversed after treatment with Shenkangwan.Compared with C group,the levels of Uα1-MG,24h UmAlb,and the renal expressions of DcR2 protein and mRNA increased significantly （P 〈0.01 ）;however,the expressions of TRAIL and DR4 protein and mRNA（P〈0.01 ）reduced significantly and came forth about the renal pathological lesion in the DM group.These changes were all reversed and the renal pathological lesion was mitigated after treatment with Shenkangwan.Conclusion：Shenkangwan have significant renal protection on T2DMrats,its mechanisms may be the effects on expression of TRAIL system.