HPLC法同时测定活血止痛液中阿魏酸和咖啡酸的含量

Content determination of ferulic acid and caffeic acid in Huoxuezhitong solution by HPLC

目的：建立同时测定活血止痛液中阿魏酸和咖啡酸含量的HPLC方法。方法：采用Inertsil ODS-SP C18色谱柱（4.6 mm×250 mm，5μm），流动相为乙腈-0.05%磷酸溶液（17∶83），流速为1.0 mL· min-1，柱温为30℃，检测波长为320 nm，进样量为10μL。结果：阿魏酸和咖啡酸与其他杂质峰分离良好。阿魏酸在1.766~35.328μg· mL-1范围内线性关系良好（r =0.9999）；咖啡酸在0.835~16.704μg· mL-1范围内呈现良好的线性关系（r=0.9993），阿魏酸和咖啡酸的平均加样回收率分别为100.79%、101.05%，RSD分别为1.73%（n=9）、1.87%（n=9）。结论：本方法简便，测定结果准确，重复性好，可作为活血止痛液的质量控制方法。

Objective：To establish a method for simultaneous determination for the content of ferulic acid and caffeic acid in Huoxuezhitong solution. Methods：Inertsil ODS-SP C18 column （4.6 mm × 250 mm, 5μm） was used for separation. The mobile phase was consisted of acetonitrile-0.05%phosphoric acid solution （17∶83） at the lfow rate of 1.0 mL·min-1. The column temperature was 30℃. The detection wavelength was 320 nm, and the injection volume was 10μL. Results：The calibration curves of ferulic acid and caffeic acid were linear in the range of 1.766-35.328μg·mL-1 （r=0.999 9） and 0.835-16.704μg·mL-1 （r=0.999 3）, respectively. The average recovery of ferulic acid was 100.79%with RSD of 1.73%（n=9）. The average recovery of caffeic acid was 101.05%with RSD of 1.87%（n=9）. Conclusion：The method is simple, accurate and reproducible, which can be used for the quality control of Huoxuezhitong solution.