运用RPLC-ICP-MS对富硒酵母中硒形态分析

Analysis of Selenium Speciation in the Selenized Yeasts by RPLC-ICP-MS

分析了硒酵母片中硒的存在形态。采用蛋白酶解法水解酵母片中的蛋白质部分,并提取出构成酵母蛋白质的各种氨基酸成分,利用反相离子对色谱与电感耦合等离子质谱联用方法对其中的含硒氨基酸进行测定,确定含硒氨基酸种类。通过与含硒标准化合物在色谱柱上的保留时间比较,确定蛋白酶解液中主要的含硒氨基酸为硒代蛋氨酸。蛋白酶解提取手段和反相离子对色谱与电感耦合等离子质谱联用的检测技术,适合于酵母中的硒形态分析。

The aim of this study is to detect the selenium speciation in the selenized yeasts. For this,to hydrolyze the proteins in the selenized yeasts through enzymatic digestion and to extract various amino acids composed the proteins in the yeats,the selenized amino acids were mensurated by reverse phase ion pair liquid chromatography and inductively coupled plasma and mass spectrometer（RPLC-ICP-MS） ,then the species of the selenized amino acids were confirmed. It was found that Selenomethionine was confirmed primary speciation of selenized amino acids in thesolutions of proteins hydrolyzed by enzyme through comparing the retention time of the amino acids with standard selenized compounds in the chromatography column. Conclusion：The method to extract the amino acids from RPLC-ICP-MS were suitable proteins hydrolyzed by the enzyme and determination technology of for analysis of selenium speciation in the selenized yeasts.