某矿区尘肺结核与单纯肺结核分离株的基因型比较

GENOTYPE COMPARATIVE ANALYSIS OF ISOLATES OF SILICOTUBERCULOSIS AND SIMPLE TU-BERCULOSIS IN A CERTAIN MINING AREA

目的比较某矿区尘肺结核与单纯肺结核分离株的基因型差异。方法 42株结核分枝杆菌分离自某矿区的尘肺结核和肺结核患者,其中尘肺结核16株,单纯肺结核26株。采用MIRU技术和BioNumerics 3.0聚类分析软件进行基因分型和成簇性分析。结果 12个MIRU位点多态性分型结果表明,尘肺结核与单纯肺结核分离株在第26、40MIRU位点的基因多态性较高,聚类分析结果证实两者的分离株具有不同的成簇性特征。结论提示煤工尘肺结核患者中流行的结核杆菌可能具有独立性。

Objective To compare the genotype discrepancy of the silicotuberculosis and simple tuberculosis isolates in coal miners. Methods This research isolated 42 strains of Mycobacterium tuberculosis from tuberculosis patients in a certain mining area, among which 16 were silicotuberculosis strains and 26 were simple tuberculosis strains. The polymorphism of 12 MIRU - VNTR loci tandem duplication units was confirmed using PCR products. BioNumerics 3.0 cluster analysis software was used to divide clinical isolates into clustering and non - clustering. Results The polymorphism typing results of 12 MIRU loci showed that the gene polymorphism of the silicotuberculosis and simple tuberculosis isolates at 26 and 40 MIRU loci was higher. The duster analysis showed that the isolates had different cluster characteristics. Conclusion This finding indicates that tubercle bacillus, which is prevalent in silieotuberculosis patients, may have independent clustering characteristics.