响应面法优化灰树花发酵全液多糖提取工艺及其抗肿瘤活性

Optimization of Microwave-Assisted Extraction of Polysaccharides from the Fermentation Broth of Crifola frondosa by Response Surface Methodology and Antitumor Activity of the Extracted Polysaccharides

研究高效提取灰树花发酵全液多糖的工艺及其抗肿瘤活性。以新鲜的灰树花发酵全液为材料，采用不同溶液pH值、微波功率、微波处理时间、热水浸提时间的单因素试验和响应面法优化微波辅助提取灰树花发酵全液多糖工艺；通过建立荷瘤小鼠模型，对灰树花发酵全液多糖的抗肿瘤活性进行研究。结果表明：微波辅助提取灰树花发酵全液多糖的最佳工艺条件为溶液pH7．0、微波功率570W、微波时间4．5min、热水浸提时间2h。此工艺条件下，灰树花发酵全液多糖得率为7．19g／L，比优化前提取效率提高24．39％。体外抗肿瘤实验表明，灰树花发酵全液多糖对小鼠S180肉瘤的抑制率为26．62％-63．01％，并可显著提高小鼠的胸腺指数和脾指数。

The microwave-assisted extraction of polysaccharides from the submerged fermentation broth of Grifola frondosa and the anti-tumor activity of the extracted polysaccharides was evaluated in a tumor-bearing mouse model. Different extractions parameters including solvent pH, microwave power, treatment time and extraction time were optimized using response surface methodology. The optimum extraction conditions that provided maximum yield of polysaccharides of 7.19 g/L, which was 24.39% higher than before the optimization were found as follows： solvent pH of 7.0, microwave power of 570 W, treatment time of 4.5 min and hot water extraction time of 2 h. Furthermore, the extracted polysaccharides not only had anti-tumor activity against mouse S180 sarcoma cells as indicated by its inhibition on the S180 sarcoma cell- derived tumor growth by 26.62%-63.01%, but also significantly increased both thymus index and spleen index in mice.