UPLC法评价多种人参提取物中人参皂苷的含量

Determination of Eight Ginsenosides in Ginseng Extracts by UPLC

利用超高效液相色谱法测定用于保健食品原料的人参提取物中8种人参皂苷的含量，并对测定的13个厂家的44批人参提取物中人参皂苷的含量进行评价。方法采用WatersACQUITYUPLCBEHC18（50mm×2．1mm，1．7μm）色谱柱，柱温30℃，乙腈．水为流动相梯度洗脱，流速0．5mL／min，203nm波长处检测。结果表明：8种人参皂苷在15min内基线分离，线性良好（r=0．9990-0．9997）。最低检出量为0．47～1．96ng。回收率在95．0％～110．0％。该方法快速简便、稳定可靠，可作为人参提取物中8种人参皂苷含量的快速测定方法。

A method for rapid determination of 8 ginsenosides in ginseng extracts of different sources using ultra performance liquid chromatography （UPLC） was established. The content of ginsenosides in 44 batches of ginseng extracts from 13 sources was analyzed and evaluated. The separation was performed on a Waters ACQUITY UPLC BEH C18 column （50 mm×2.1 mm, 1.7 μm） at 30 ℃ at a flow rate of 0.5 mL/min through gradient elution with acetonitrile-water mixture and detection at a wavelength of 203 nm. Eight ginsenosides were well separated within 15 min with correlation coefficients of 0.9990-0.9997. The detection limits for ginsenosides were 0.47-1.96 ng with recoveries ranging from 95.0% to 110.0%. The method was simple, rapid, and reliable, and thus, was suitable for the rapid determination of 8 ginsenosides in ginseng extracts from different sources.