用RP-HPLC法测定七厘散中羟基红花黄色素A的含量

Content determination of hydroxysafflower yellow A in Qili san by RP-HPLC method

目的：建立RP-HPLC法测定七厘散中羟基红花黄色素A的含量。方法：采用Mucleodur C18色谱柱（250 mm×4.6 mm，5 μm）；流动相：乙腈-0.1%磷酸溶液 （11∶89）；流速：1.0 ml/min；检测波长：403 nm；柱温：35 ℃。结果：羟基红花黄色素A保留时间约为19 min，与相邻峰的分离度〉1.5。羟基红花黄色素A在8.0～160.0 μg/ml范围内线性关系良好，回归方程：Y=0.016 11 X＋1.217（r=0.999 9）。平均加样回收率为99.5%，RSD为0.94%。结论：该方法准确、灵敏、重现性好，可用于七厘散中羟基红花黄色素A的含量测定。

Objective：To establish a RP-HPLC method for determination of hydroxysafflower yellow A content in Qili san. Methods： The separation was performed on a Mucleodur C18 column with the mobile phase acetonitrile-0. 1% phosphoric acid （11 ± 89） at a flow rate of 1.0 ml/min. The detection wavelength was 403 nm and column temperature was 35℃. Results：The retention time of hydroxysafflower yellow A was about 19 rain. The resolution with the adjacent peak was more than 1. 5. Hydroxysafflower yellow A presented a good linearity within the range of 8.0 160.0μg/ml, and the regression equation was Y=0. 016 11 X＋1. 217 （r=0. 999 9）. The average recovery of hydroxysafflower yellow A was 99.5% and RSD was 0.94%. Conclusion：This method is accurate, sensitive and reproducible. It can be used for content determination of hydroxysafflower yellow A in Qili san.