子宫腺肌病病灶细胞的培养与鉴定

Primary culture and identification of adenomyosis cell

目的原代培养及鉴定子宫腺肌病病灶细胞，为研究子宫腺肌病发病机制与药效学提供新的理想实验模型。方法采用胶原酶消化法对人子宫腺肌病细胞进行原代培养，并采用免疫细胞化学技术鉴定。结果成功培养了6例子宫腺肌病细胞并均经免疫细胞化学法鉴定证实，成功率为75％；第1代与第8代细胞的生长曲线基本一致。结论胶原酶消化法培养的子宫腺肌病细胞性能稳定，后续研究可选择3～6代细胞作为药物干预模型，细胞传代后4—5天加药物干预，选择24小时作为药效学作用的检测时间点。

Objective To provide an ideal experimental model for the study of pathogenesis and pharmacodynamic of adenomyosis （AD） by primary culture and identification of AD cells. Methods AD cells were digested with collagenase in primary culture and identified by immunocytochemistry. Results Six AD cells were successfully cuhured and were identified by immunocytochemistry, and the success rate was 75%. The growth curve of cells of the first generation and 8th generation was almost the same. Conclusion The quality of cultured AD cells by collagenase digestion is stable. The cells of 3rd to 6th generation can be selected to be the model for drug intervention. The drug intervention time is in 4-5 days after cells passage, and 24h is taken as an assay time for pharmaceutical efficacy.