氯化钠胁迫对雷竹根系活力和细胞膜透性的影响

Root activity and cell membrane permeability in Phyllostachys violascens with NaCl stress

为了研究雷竹Phyllostachys violascens在氯化钠胁迫下的耐盐能力,通过不同质量浓度氯化钠处理的1年生盆栽模拟试验,对氯化钠胁迫对雷竹根系活力和细胞膜透性的影响进行了分析。结果表明:氯化钠胁迫下根系活力对照最高为12.83μg·g-1·h-1,其次是3.0 g·L-1氯化钠处理为10.80μg·g-1·h-1,与对照没有显著性差异(P=0.326),5.0 g·L-1氯化钠处理最低,为7.22μg·g-1·h-1,与对照有显著性差异(P=0.008)。在氯化钠胁迫的前期雷竹对胁迫有一定的应激能力,根系能够保持较高的活力,但随着氯化钠胁迫的加重和时间的推移,根系受到损伤,活力下降;细胞膜透性随氯化钠胁迫质量浓度的变化而变化,对照处理的细胞膜透性最小为6.03%,1.0和3.0 g·L-1氯化钠处理的细胞膜透性基本一致,分别为6.97%和6.93%,而5.0 g·L-1氯化钠处理的细胞膜透性明显上升为8.75%,在氯化钠胁迫前期细胞膜透性的变化比较剧烈,而后期就比较平稳。

In order to expand Phyllostachys violascens planting area, we try to find the reason of Ph. violascens fail to grow well even decay and death in the saline-alkali land, therefore salt tolerance in Phyllostachys violascens with NaCl stress is studied, root activity and cell membrane permeability were studied in a pot culture experiment with a different concentration of NaCl （mass concentration, respectively 0, 1.0 , 3.0, 5.0 g.L^-1） treatments：（1） 12.83 μg.g^-1.h^-1 treated with 0 g.L^-1 NaCl solution, （2） 9.4 μg.g^-1.h^-1 treated with 1.0 g.L^-1 NaCl solution, （3） 10.80 μg.g^-1.h^-1 treated with 3.0 g.L^-1 NaCl solution, （4） 7.22 μg.g^-1.h^-1 treated with 5.0 g. L^-1 NaCl solution. Results showed that Treatment 1 made roots the most active, Treatment 3 was not significantly different from the control （P = 0.326）, and Treatment 4 was signifibantly different from the control （P = 0.008）. Ph. violascens showed some adaptability under NaCl stress with higher root activity in the earlier stages. However, the root system was damaged, and vigor declined over time. Cell membrane perme- ability of the Treatment 1 was 6.03%; there were no significant differences with Treatments 2 （6.97%）or 3 （6.93%）, but Treatment 4 was greater at 8.75%. Overall, changes in cell membrane permeability were greater in the early stages of NaCl stress treatment and more stable in the later stages.