骨桥蛋白在胸腺瘤组织中的表达及临床病理意义

Expression and Clinicopathological Significance of Osteopontin in Thymoma

目的检测骨桥蛋白(osteopontin,OPN)在胸腺瘤组织中的表达情况并探讨其临床病理意义。方法应用免疫组织化学SP法检测OPN在60例胸腺瘤和18例正常胸腺组织中的表达情况,结合病例资料及胸腺瘤Masaoka分期、胸腺瘤WHO分型,比较OPN在胸腺瘤与正常胸腺组织、不同胸腺瘤Masaoka分期、不同胸腺瘤WHO分型的表达差异。结果 OPN在胸腺瘤组织中阳性颗粒的平均灰度和阳性单位值显著高于正常胸腺组织(P<0.05);OPN在胸腺瘤不同Masaoka分期间阳性颗粒的平均灰度和阳性单位值差异均有统计学意义(均P<0.01);OPN在胸腺瘤不同WHO分型间阳性颗粒的平均灰度和阳性单位值差异均有统计学意义(均P<0.01)。结论 OPN与胸腺瘤的形成及胸腺瘤的恶性程度、侵袭性有关。OPN的表达可能作为胸腺瘤临床诊断和患者预后判断的一个重要参考指标。

Objective To detect the expression of osteopontin（OPN）in thymoma, and to investigate its clinicopathological significance.Methods The expression of OPN in 60 thymoma tissues and 18 normal thymus tissues were detected by using immunohistochemical SP method. In addition, OPN expression was compared between thymoma tissues and normal thymus tissues, among different Masaoka stages and among different WHO types. Results Compared with normal thymus tissues, the average gray level of positive particles and positive unit values of OPN significantly increased in thymoma tissues （P〈0.05）. Moreover, there were significant differences in the average gray level of positive particles and positive unit values of OPN among different Masaoka stages, as well as among different WHO types （P〈0.01）. Conclusion OPN is remarkably correlated with the occurrence, malignancy and invasion of thymoma. The expression of OPN may be used as an important reference index in the clinical diagnosis and prognosis of thymoma.