摘要
目的:探讨内毒素(LPS)体外刺激对人外周血细胞高迁移率族蛋白B1(HMGB1)及肿瘤坏死因子(TNF)-α、白介素(IL)-6和IL-10分泌的影响.方法:采集20例健康志愿者外周血,与培养基等体积混合后体外培养,给予不同浓度LPS(100 ng/ml,500 ng/ml)刺激,分别于刺激后0、2、6、12、24 h收集细胞培养上清液,ELISA法检测其HMGB1和TNF-α、IL-6、IL-10的含量.结果:体外LPS刺激诱导外周血细胞分泌HMGB1增加,12 h后表达持续升高(P〈0.01),分泌水平呈明显时间依赖性;TNF-α、IL-6产生亦呈时间、剂量依赖性,分别于24 h和6~12 h达分泌高峰(P〈0.01);IL-10表达量均较低.结论:TNF-α、IL-6为早期炎症介质,HMGB1为介导内毒素所致迟发死亡的重要晚期炎症介质,它们在脓毒症的发生、发展中可能扮演重要角色.
Objective:To observe the effect of lipopolysaccharide (LPS) stimulation on secretion of high mobility group box-1 protein (HMGB1), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6) and IL-10 from cells of human peripheral blood in vitro. Methods: Peripheral blood samples were collected from 20 healthy volunteers and cultured with the equal volume of cell culture medium in vitro. After treatment with LPS (100 ng/ml or 500 ng/ml) for 0, 2, 6, 12, or 24 hours, culture supernatants were collected and levels of HMGB1, TNF α, IL-6 and IL-10 were measured with ELISA. Results: A significant release of HMGB1 in human blood was observed after cultured with LPS for 12 hours (P(0. 01), showing a time-dependent response manner. Similarly, production of TNF-α and IL-6 peaked at 24 hours and 6-12 hours in a dose-dependent manner after stimulation with LPS (P〈0. 01). Expression of IL-10 remained in low level after stim- ulation with LPS in various doses. Conclusions: HMGB1 is a late inflammatory factor in inducing the late death as a result of endotoxin challenge. TNF-α and IL-6 are important early pro-inflammatory cytokines acting as the motivator in the de velopment of sepsis. Both early and late pro-inflammatory cytokines appear to be involved in the development of sepsis.
出处
《感染.炎症.修复》
2013年第3期136-139,共4页
Infection Inflammation Repair
基金
国家自然科学基金项目(81071545
30971192
81130035
81121004)
国家重点基础研究发展计划项目(2012CB518102)
全军"十二五"计划重大项目(AWS11J008
BWS12J050)