摘要
本研究通过正交试验优选了紫花苜蓿中芦丁的提取工艺,以510nm最大吸光度进行可见光谱检测,测定芦丁含量,并对两种供试菌进行了体外抑菌活性测定。结果表明,通过单因素试验确定的各因素影响能力大小为:料液比>乙醇浓度>超声提取时间,含量最高为10.04mg/g;大肠埃希氏杆菌的MIC为0.40mg/mL,金黄色葡萄球菌的MIC为0.70mg/mL。
The extraction method of rutin from alfalfa was selected through an orthogonal test,the content determination of rutin was test 510 nm maximum spectrophotometry visible spectrum and the antibacterial activity for two selected bacteria in vitro was determination.The results showed that the order for the influence of various factors was Liquid to solid ratio ＞ Ethanol concentration ＞ Ultrasonic extraction time,the highest content was 10.04 mg/g,the MIC for E.coli and S.aureus were 0.40 mg/mL and 0.70 mg/mL respectively.
出处
《中国奶牛》
2013年第20期25-27,共3页
China Dairy Cattle
基金
甘肃省技术研究与开发专项计划(0912TCYL004)基金资助
关键词
紫花苜蓿
芦丁
抗菌
Alfalfa
Rutin
Antibacterial
