花粉提取物对D-半乳糖胺盐酸盐致大鼠肝损伤的保护作用

Effect of Pollen Extracts on Hepatic Injury of Rats Induced by D-Galactosamine

目的采用溶剂分级萃取,得到油菜蜂花粉的5个提取组分,通过D-半乳糖胺盐酸盐致大鼠急性肝损伤实验,考察5个提取组分对肝损伤大鼠的保护作用。方法实验分为7组:空白对照组、D-半乳糖胺盐酸盐模型组、花粉提取物A、B、C、D、E组。连续给药10 d后,除空白对照组外,其余各给药组动物腹腔注射10%D-半乳糖胺盐酸盐溶液制备肝损伤模型。测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)的活性以及肝组织中白介素-6(IL-6)及肿瘤坏死因子α(TNF-α)的水平。结果花粉提取物A组AST、ALP的活性以及IL-6、TNF-α水平明显低于模型组;花粉提取物B、E组ALT、AST、ALP的活性以及TNF-α水平明显低于模型组。结论花粉提取物A、B、E组能缓解D-半乳糖胺所致的肝损伤,是油菜蜂花粉拮抗D-半乳糖胺盐酸盐所致大鼠肝损伤的活性组分,其机制可能与降低炎性介质TNF-α、IL-6的水平有关。

Objective To observe the protective effect of five constituents of bee collected from rape pollen on rats with hepatic injury caused by D-galactosamine hydrochloride. Methods The experiment was divided into seven groups： control group,D-galactosamine hydrochloride model group,pollen extract A,B,C,D,E groups. After successive administration for 10 days,these groups except the blank control group received intraperitoneal injection of 10% D-galactosamine hydrochloride solution to induce a liver injury model. The activity of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,alkaline phosphatase（ALP） in serum and the level of interleukin-6（IL-6） and tumor necrosis factor α（TNF-α） in liver tissue were measured. Results Compared with the model group,pollen extract A significantly reduced the activity of AST,ALP and the level of IL-6 and TNF-α,while pollen extract B,E could significantly reduce the activity of ALT,AST,ALP and the level of TNF-α. Conclusion Pollen extract A,B,E can reduce the biochemical parameters of D-galactosamine-induced liver injury and have a certain protective effect on the liver of rats. The mechanism may be related to its anti-inflammatory function as shown by the reduced level of IL-6 and TNF-α in liver tissue.