HPLC法测定红花益气养血胶囊中西红花苷-Ⅰ和西红花苷-Ⅱ的含量

Determination of Crocin-Ⅰ and Crocin-Ⅱ in Honghuayiqiyangxue Capsules

目的建立HPLC法测定红花益气养血胶囊中西红花苷-Ⅰ和西红花苷-Ⅱ的含量。方法色谱柱：Hypersil ODS2C18柱（150 mm×4.6 mm,5μm）,流动相：甲醇-水（48∶52）,检测波长：440 nm;流速：1.0 ml·min-1;柱温：室温。结果西红花苷-Ⅰ在12~45μg·ml-1的浓度范围内线性关系良好（r=1.0000）,平均回收率为99.44%,RSD为1.19%（n=6）;西红花苷-Ⅱ在6.4~24μg·ml-1的浓度范围内线性关系良好（r=0.9999）,平均回收率为98.79%,RSD为1.38%（n=6）。结论本法简便、快速、准确,可用于红花益气养血胶囊中西红花苷-Ⅰ和西红花苷-Ⅱ的含量测定。

Objective To establish an HPLC method for the content determination of crocin-Ⅰand crocin-Ⅱin Honghuayiqiyangxue capsules. Methods A Hypersil ODS2 C 18 column（150 mm × 4. 6 mm,5 μm） was used with methanol-water（48∶ 52） as the mobile phase. The detection wavelength was 440 nm. The flow rate was 1. 0 ml·min-1. Results The calibration curves of crocin-Ⅰand crocin-Ⅱwere linear in the range of 1245 μg·ml-1（r =1. 0000） and 6. 424 μg·ml1（r =0. 9999）,respectively. The average recovery was 99. 44%（RSD =1.19%,n =6） for crocin-Ⅰand 98. 79%（RSD = 1. 38%,n = 6） for crocin-Ⅱ. Conclusion HPLC can determine the content of crocin-Ⅰ and crocin-Ⅱin Honghuayiqiyangxue capsules efficiently and accurately.