SPE-HPLC法测定鼻炎灵片中黄芩苷与欧前胡素的含量

Determination of Baicalin and Imperatorin in Biyanling Pills by SPE-HPLC

目的建立SPE-HPLC测定鼻炎灵片中的黄芩苷、欧前胡素的含量测定方法。方法采用HPLC法,色谱柱：Diamonsil C18（5μm,250 mm×4.6 mm）,流动相：甲醇（A）-0.1%磷酸溶液（B）梯度洗脱,检测波长：280 nm;流速1.0ml·min-1,进样量10μl。结果黄芩苷在1.311~131.1μg·ml-1范围内线性关系良好（r=0.9993）,平均加样回收率为99.8%,RSD为0.1%（n=6）;欧前胡素在1.003~100.3μg·ml-1范围内具有良好的线性关系（r=0.9994）,平均加样回收率为99.0%,RSD为0.6%（n=6）。结论该方法简便快捷,准确、重复性好,灵敏度高,可用于建立鼻炎灵片的质量控制。

Objective To establish an SPE-HPLC method for content determination of Biyanling pills. Methods Diamonsil C 18（5 μm,250 mm ×4. 6 mm） was used. The mobile phase was methanol-0. 1% phosphoric acid（gradient elution） at a flow rate of 1. 0 ml·min-1. The detection wavelength was set at 280 nm. Results The linear range of baicalin was 1. 311131. 1 μg·ml-1（r =0. 9993）. The average recovery was 99. 8%（n =6,RSD = 0. 1%）. The linear range of imperatorin was 1. 003100. 3 μg·ml-1（r =0. 9994）. The average recovery rate was 99. 0%（n = 6,RSD = 0. 6%）. Conclusion This method is simple,accurate and reproducible,which can be used for quality control of Biyanling pills.