舒洛地特对高糖环境中大鼠肾小球系膜细胞CD36抗原表达的抑制作用研究

Inhibitory Effects of Sulodexide on Expression of CD36 in Rat Glomerular Mesangial Cells Cultured in High Glucose

目的:观察舒洛地特对高糖培养基中大鼠肾小球系膜细胞CD36抗原表达的抑制作用,探讨舒洛地特对肾脏的保护作用。方法:培养大鼠肾小球系膜细胞24 h后,将培养的细胞分为对照组(C组,普通培养基,含5.6 mmol·L^(-1)葡萄糖),甘露醇组(M组,24.2 mmol·L^(-1)甘露醇+C组),高糖组(H组,30 mmol·L^(-1)高糖MEM培养基)、舒洛地特干预组(S组,H组+1.0 LRU·ml^(-1)舒洛地特)。培养24 h后,RT-PCR和Western blotting法检测各组CD36 mRNA及蛋白的表达。结果:对照组和甘露醇组CD36mRNA(0.24±0.07和0.21±0.06)及蛋白(0.26±0.08和0.22±0.07)的表达差异无统计学意义(P>0.05),高糖组CD36 mRNA(0.62±0.11和0.24±0.07)及蛋白(0.83±0.23和0.26±0.08)表达显著高于对照组,差异有统计学意义(P<0.05),舒洛地特干预组CD36 mRNA(0.36±0.13和0.62±0.11)及蛋白(0.42±0.15和0.83±0.23)表达显著低于高糖组,差异有统计学意义(P<0.05)。结论:舒洛地特可抑制高糖环境中大鼠肾小球系膜细胞CD36抗原的表达,可能是舒洛地特发挥肾脏保护作用的机制之一。

Objective：To investigate the inhibitory effects of sulodexide on the expression of CD36 induced by high glucose in rat glomerular mesangial cells.Method：The mesangial cells were cultured for 24 hours and then divided into 4 groups;the control group（C,5.6 mmol？L-1 glucose）,mannitol group（M,24.2 mmol？L-1 mannitol ＋ group C）;high glucose group（H,30 mmol？L-1 glucose） and sulodexide group（S,group H ＋ 1.0 LRU·ml-1 sulodexide）.The expression of CD36 were detected by RT-PCR and Western blotting.Result：No significant difference in CD36 was found between group C and group M with mRNA of（0.24 ±0.07） and（0.21 ±0.06）（P＆gt;0.05）and protein level of（0.26 ±0.08） and（0.22 ±0.07）（P ＆gt;0.05）,respectively;Comparing with that in group C,the expression of CD36 in group H was increased markedly with mRNA of（0.62 ±0.11） and（0.24 ± 0.07）（P ＆lt; 0.05） and protein level of（0.83 ±0.23） and（0.26 ±0.08）（P ＆lt;0.05）,respectively,and that was decreased markedly in group S compared with that in group H with mRNA of（0.36 ±0.13） and（0.62 ±0.11）（P ＆lt;0.05）and protein level of（0.42 ±0.15） and（0.83± 0.23）（P ＆lt;0.05）,respectively.Conclusion;Sulodexide can inhibit the expression of CD36 in rat glomerular mesangial cells under high glucose condition,which may be one of the mechanisms of kidney protective function.