HPLC法测定微米大黄炭中没食子酸的含量

Determination of Gallic Acid in Micron Rhubarb Charcoal by HPLC

目的:建立HPLC法测定微米大黄炭中没食子酸含量。方法:采用Fortis universil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇:0.1%磷酸溶液(12:88);检测波长:273 nm;流速:0.9 ml·min^(-1)。结果:没食子酸回归方程为Y=31.93X+2.61(r=0.999 9),其浓度线性范围6.6~66.0μg·ml^(-1)。平均回收率为101.35%,RSD=1.16%(n=6)。结论:测定方法简便、准确、重复性好,可用于微米大黄炭中没食子酸的含量测定。

Objective：To establish an HPLC method for the determination of gallic acid in micron rhubarb charcoal.Method：A Fortis universil C18 column（250 mm×4.6 mm,5 μm） was used.The mobile phase was methanol-0.1%phosphate acid（12：88）,the detection wavelength was 273nm,and the flow rate was 0.9 ml·min-1.Result：The regression equation of gallic acid was Y = 31. 93X＋2.61,r = 0.999 9.The linear range of gallic acid was 6.6-66.0μg？ml-1,the average recovery was 101.35%with RSD of 1.16%（n=6）.Conclusion：The method is simple,reliable and accurate,and can be used in the content determination of micron rhubarb charcoal.