摘要
目的：建立桃仁中苦杏仁苷灵敏度高、适用性强的高效液相色谱测定新方法，并对收集于新疆地区的41批代表性药材进行含量测定。方法：样品采用索氏提取器脱脂、加入70％甲醇回流的方法灭酶和提取，色谱柱为SymmetryC18（4．6mm×150mm，5μm），以水-乙腈为流动相梯度洗脱，流量1mL·min^-1，柱温35℃，检测波长210nm。结果：方法学验证各项指标均符合要求，41批新疆桃仁中苦杏仁苷含量在2．05％-3．62％范围内，平均含量为3．04％±0．29％。结论：所建立测定方法简便、稳定、易行，可用于新疆桃仁中苦杏仁苷含量测定和质量控制。
Objective： To improve the HPLC method for determination of amygdalin in Persicae Semen, and the improved method was applied for evaluation of 41 batches representative herbs collected from Xinjiang. Method： The samples of enzyme inactivation were obtained by degreasing with Soxhlet extractor and refluxing with 70% methanol; Symmetry Cls （4. 6 mm± 150 mm, 5 μm） column was adopted with gradient elution of acetonitrile （A） and water （B） at flow rate 1.0 mL · min-1; The column temperature was 35℃ and the wavelength was set at 210 nm. Result： All the validated parameters met with the requirements for assay. The amygdalin content of Persicae Semen ranged between 2. 05% and 3.62% , and the average was 3.04% ± 0. 29%. Conclusion： The improved method is simple, stable, feasible and reproducible. It can be used for the quality control of Persicae Semen originating from Xinjiang.
出处
《中国药品标准》
CAS
2013年第6期433-436,共4页
Drug Standards of China
基金
新疆乌鲁木齐科技攻关计划(G121120002)
创新基金(CX11125W)
