大鼠心肌梗死后血清脑钠肽、内皮素-1和一氧化氮的变化及缬沙坦的干预作用

Evolvement Laws of Serum Brain Natriuretic Peptide, Entothelin -1 and Nitric Oxide in Rats after Myocardial Infarction and Effects of Valsartan on them

目的通过动物实验发现急性心肌梗死后大鼠脑钠肽（BNP）、内皮素-1（ET-1）和一氧化氮（N0）的变化规律，以及缬沙坦对这些体液因子的干预作用。方法Wistar大鼠90只，经麻醉后行冠脉结扎处理制作心肌梗死模型，术后存活的大鼠分为①对照组（Sham，n=30）；②心肌梗死组（AMI，n=30）；③心肌梗死＋缬沙坦组（AMI＋valsartan，n=30）。心肌梗死＋缬沙坦组于术后将缬沙坦行干预治疗。术后开始计时，于6，12，24h和3，7，30天分别抽大鼠静脉血1ml，测定BNP，ET-1和N0指标，观察各组之间的差异。结果各组间血清BNP，ET-1和N0水平在每个时间点均有明显差异（各P值均〈O．05），其中BNP和ET-1有单纯心肌梗死组〉心肌梗死＋缬沙坦组〉对照组的大小关系，而N0有单纯心肌梗死组〈心肌梗死＋缬沙坦组〈对照组的大小关系。结论血清BNP，ET-1升高和N0的降低参与了AMI后病情演变过程。缬沙坦能改善AMI后血清BNP，ET-1升高和N0的降低程度，对AMI后病情变化有益。

Objective To find the evolvement laws of brain natriuretic peptide （BNP）, entothelin-1 （ET-1） and nitric oxide （NO） serum levels in rats after acute myocardial infarction and observe the effects of the valsartan on them. Methods 90 male wister rats were randomly divided into three groups.. （1）sham group （n= 30） ,（2）AMI group （n= 30）, （3）AMI＋ valsartan group （n= 30）. Rats in the AMI＋ valsartan group were given the valsartan. Ratt s serum concentrations of BNP, ET-1 and NO were measured at all times （6,12,24 h and 3,7,30 d） to compare the differences in three groups. Results At every time, there all was a sginificant difference in the blood serum levels of BNP,ET-1 and NO from AMI group,AMI＋ valsartan group and sham group. Compared with AMI＋ valsartan group, the blood serum levels of BNP and ET-1 at every time in AMI group was higher,but in sham group was smaller （P〈0.05）. Contrarily, compared with AMI＋ valsartan group, the blood serum levels of NO at every time in AMI group was smaller,but in sham group was higher （P〈0. 05）. Conclusion （1） This showed that the increase of BNP and ET-1 blood serum levels and the decrease of NO blood serum level participated in the progress of AMI. （2）This showed that the valsartan could lessen the increase of BNP and ET-1 blood serum levels and the decrease of NO blood serum level and was beneficial to the progress of AMI.