摘要
目的 :建立高效液相色谱法测定肠必清肠溶颗粒剂中番泻苷A、B含量的方法。方法 :以C18 柱为分析柱 ,以50 %乙腈 (含0 25mmol/L十六烷基三甲基溴化铵和0 25mmol/L磷酸氢二钠 ,每200ml加入冰醋酸75μl)为流动相 ,流速为1ml/min ,检测波长为270nm。结果 :在0 25μg/ml~80 0μg/ml浓度范围内呈线性关系 (番泻苷A :r=0 9997 ;番泻苷B :r=0 9997) ,番泻苷A、B测定方法重现性较好 (RSD<2 % )的精密度均<2 % ,回收率均>98 %。结论 :本方法简单、准确、重现性好 。
OBJECTIVE:To estoblish a method of determination of the contents of sennoside A and sennoside B in Chang Bi Qing enteric-coated granules with HPLC.METHODS:The analytical column was C18.The mobile phase was 50% acetonitrile(containing 0.25mmol/L cetrimid and 0.25mmol/L Na2HPO475μl acetic acid in 200ml).The flow rate was 1.0ml/min.The detecting wave-length was 270nm.RESULTS:The line was well in the range of 0.25μg/ml~80.0μg/ml(sennoside A:r=0.9 997;sennoside B:r=0.9 997).The average recoveries of sennoside A and sennoside B were 98.14% and 98.07% respectively and their repeatabilities were good(standard derivations<2%).CONCLUSION:The method was simple,accurate,reproducible and can be used for quality control of the preparation.
出处
《中国药房》
CAS
CSCD
2000年第4期153-155,共3页
China Pharmacy
关键词
高效液相色谱
肠必清肠溶颗粒
含量
测定
中药
HPLC
Chang Bi Qing enteric-coated granule
sennoside A
sennoside B
assay of content