糖尿病肾病早期生化诊断研究

Research on early biochemical diagnosis for diabetic nephropathy

目的探讨尿微量清蛋白(mALB)、β2-微球蛋白(β2-MG)、胱抑素C(Cys-C)水平对2型糖尿病患者早期肾损伤的诊断价值。方法采用速率免疫比浊法检测尿mALB,采用胶体金颗粒免疫比浊法测定Cys-C,采用乳胶增强免疫比浊法检测β2-MG水平。结果糖尿病患者尿mALB、血清Cys-C、β2-MG水平均显著高于对照组,差异有统计学意义(P<0.05)。糖尿病患者尿mALB、血清Cys-C、β2-MG阳性检出率比较:尿mALB显著高于血清Cys-C、β2-MG阳性检出率,阳性检出率由高到低分别为尿mALB、血清Cys-C、β2-MG,差异有统计学意义(P<0.01)。结论 2型糖尿病存在不同程度肾脏损害,尿mALB、血清Cys-C、β2-MG对肾脏早期损害有较好的诊断作用;尿mALB最能反映糖尿病肾病早期肾功能损害,可成为判断早期肾功能损害程度的敏感指标。

Objective To investigate diagnostic value of the urinary microalbumin albumin （mALB） ,β2-micro-globulin（β2-MG） and cystatin C（Cys-C） levels to early renal injury of patients with type 2 diabetes .Methods Rate of immun turbidimetry was used to detect mALB in urinate ,colloidal gold particles immune turbidimetric method was used to detect Cys-C ,latex-enhanced turbidimetric immunoassay was used to detect β2-MG levels .Results The ser-um Cys-C ,β2-MG of the diabetic patients with urinary albumin were significant higher than those of the control group （P〈0 .05） ,Compared the positive rates of mALB ,Cys-C andβ2-MG ,mALB was significant higher than other indica-tors（P〈0 .01） .Conclusion There are varying degrees of renal impairment in type 2 diabete .These indicators that mALB in urinate ,Cys-C、β2-MG in serum are sensitive to early renal damage ,especially mALB in urinate .