EH-2060B型全自动尿沉渣分析仪的临床应用

Clinical application of EH-2060B automatic urinary sediment analyzer

目的探讨使用EH-2060B型全自动尿沉渣分析仪在临床尿分析检查中的应用价值,分析各种尿液分析方法所致结果差异的原因及解决方法。方法按中国临床实验室标准化委员会有关规定和《尿沉渣检查标准化的建议》要求随机收集1 912例尿液标本,使用EH-2060B型尿沉渣分析仪和干化学法进行红细胞(RBC)、白细胞(WBC)等有形成分的分析。结果以尿沉渣镜检法为基础进行评价,干化学法检测RBC、WBC假阴性率为16.8%、22.8%,假阳性率为11.5%、5.7%,均高于EH-2060B分析仪假阴性率15.1%、17.2%,假阳性率3.2%、2.1%,两种方法除了尿RBC的假阴性率差异无统计学意义(P>0.05),其余指标差异均有统计学意义(P<0.05)。以镜检法为参考方法,EH-2060B尿沉渣仪检测RBC、WBC总符合率及一致性检验Kappa值均高于干化学法。干化学法与尿沉渣仪联合检测,RBC假阴性率为2.0%,WBC假阴性率为3.9%。结论与干化学法比较,EH-2060B型尿沉渣分析仪更方便、快捷、结果可靠,但也存在一定局限性,不能完全替代人工镜检,只能作为筛选指标,两种方法应联合检测,互为补充,并以显微镜镜检法为金标准,提高尿液有形成分的检出率。

Objective To investigate the application value of EH-2060B automatic urinary sediment analyzer in clinic ,to find out the reason what induce results differing from other methods and the solution .Methods A total of 1 912 urine samples were collected randomly under the requirements of CCCLS （China Committee for Clinical Labora-tory Standards） and ＂Proposal of standardization for the Urine Sediment Exam＂,then analyzed the erythrocyte and leucocyte by urine dipsticks analyzer and EH-2060B automatic urinary sediment analyzer respectively .Results Refer-ring to the microscopic examination ,the false negative rate and the false positive rate of analyzing the erythrocyte and leucocyte by dry chemistry（false negative rate ：16 .8% and 22 .8% ,the false positive rate ：11 .5% and 5 .7% ） were both higher than those of EH-2060B （false negative rate ：15 .1% and 17 .2% ,the false positive rate ：3 .2% and 2 .1% ） ,Except the false negative rate of the analyzing the erythrocyte ,there were significant difference in the other index between the urine dipsticks analyzer and EH-2060B automatic urinary sediment analyzer （P〈0 .05） .To com-pare the total coincidence and consistency with manual microscope ,the testing Kappa value of EH2060-B were higher than dry chemistry .Combined dry chemistry and sediment analyzer ,the false negative rate for RBC and WBC was im-proved to 2 .0% and 3 .9% .Conclusion Compared to the urine dipsticks analyzer ,EH-2060B analyzer is rapid ,simple and of high coincidence with manual microscopy ,however ,there are certain limitations ,it can′t replace the micro-scope examination at all ,and only as the preliminary screening .These two analysis methods could be complementary each other while taking manual microscopy as the golden criterion so as to gradually raise the positive coincidence of urinary sediment .