摘要
将外植体接种于诱导愈伤组织和芽的分化培养基上 ,培养 1个周期 (30~ 40d),外植体上出现光滑的愈伤组织 .2个周期后愈伤组织上分化出芽体 .将有芽体分化的愈伤组织转入 MS+ BA0.5mg/L+ NAA0.1mg/L培养基中, 2个月左右可长成 2~ 3cm的生根小植株 .生根试管苗经炼苗后移入花盆,生长良好 .
The explants were cultured on the medium of inducing callus and differentiation bud. The calli developed on the explants after 1 period (30~ 40 days). The buds were induced on the calli cultured on the differentiation medium after 2 periods.Then the calli with buds were transfered onto MS+ BA0.5mg/L+ NAA0.1mg/L medium , the growth of buds quickened and could grow into rooting plantlets of 2~ 3cm high in about 2 months. The rooted test tube plants grew well after being transfered into flowerpots.
出处
《淄博学院学报(自然科学与工程版)》
2000年第3期68-70,共3页
Journal of Zibo University(Natural Sciences and Engineering)
基金
淄博市科委资助项目