Doehlert设计优化超声波辅助酶法提取河蚬多糖工艺研究

Application of Doehlert Matrix Design in Optimizing Ultrasonic-assisted Enzymatic Extraction of Polysaccharides from Corbicula fluminea

研究用超声波辅助酶法提取河蚬粗多糖,考察超声温度、超声时间、料液比、超声功率对多糖产量的影响,运用Doehlert matrix设计与响应曲面优化得到了最优条件下的最优产量。结果表明,超声波辅助酶法提取河蚬粗多糖最优工艺参数为超声温度82℃、超声时间35min、料液比37mL/g、超声功率270W,多糖产量达到42.7%。

Ultrasonic-assisted Enzymatic Extraction Technology was applied to extract Corbicula fluminea polysaccharides.Four independent variables were extraction temperature,ultrasound time,ratio of material to liquid and ultrasonic power.Doehlert Matrix Design and Response Surface Methodology were used to determine the optimum process parameters that could give highest extraction yield.The optimal polysaccharide yield of 42.7％ was obtained under extraction temperature 82℃,ultrasonic time 35min,ratio of material to liquid 37 mL/g and ultrasonic power 270W.