高产漆酶菌株的筛选及其对秸秆降解初探

Screening of High Laccase-Producing Strains and Studies on the Degradation of Stalk

主要对21个隶属于担子菌门的菌株进行了漆酶活性的比较。通过平板培养进行初步筛选,液体发酵的方法进行复筛,培养15 d后得到高酶活的菌株(酶活达到300U/mL以上)3株,依次是一色齿毛菌(Cerrena unicolor);薄皮干酪菌(Tyromyces chioneus)和烟管菌(Bjerkandera adusta)。研究3种菌株对秸秆纤维素、半纤维素和木质素降解的能力,实验结果表明:B.adusta和T.chioneus是较好的秸秆木质素降解菌株,对木质素降解率依次为66.13%,61.92%。

The laccase activity of 21 strains belong to basidiomycetes was compared. 3 high lacease-producing level strains were screened out using the plate cultivation and fluid fermentation method, which were Cerrena unicolor, Tyromyces chioneus and Bjerkandera adusta. The laccase activity of the strains cultivated for 15 days were above 300 U/mL. Then the degradation effect of stalk by the 3 strains was studied. The result showed the effect of lignin degrada-tion by B. adusta and T. chioneus were higher, the degradation rate were 66.13% and 61.92%.