NS-398对肝癌细胞SMMC-7721 COX-2、Survivin表达的影响

Effect of NS-398 on the expression of COX-2 and Survivin in hepatocellular car-cinoma SMMC-7721 cells

目的:观察COX-2与Survivin在SMMC-7721细胞中的表达情况及COX-2抑制剂NS-398对二者表达的影响,探讨NS-398的抗肿瘤作用机制。方法:以不同浓度的NS-398(0、25、50、75、100、150μmol/L)处理SMMC-7721细胞24、48、72小时,应用qRT-PCR检测COX-2、Survivin mRNA的表达,应用Western blot检测COX-2、Survivin蛋白的表达,应用FCM鉴定COX-2、Survivin蛋白表达。结果:COX-2与Survivin在正常生长的SMMC-7721细胞中均有较高表达,NS-398处理后,COX-2、Survivin基因和蛋白表达均显著减少,即与NS-398的作用剂量及时间呈反比。结论:NS-398可能是通过降低COX-2与Survivin的表达来实现改变细胞周期、诱导凋亡,从而抑制肿瘤的生长。

Objective ：To observe the expression of COX-2 and Survivin in hepatocellular carcinoma SMMC-7721 cells and in- vestigate the mechanism of NS-398 on inhibiting tumor cell growth. Methods： The hepatoma cell line SMMC-7721 was cultured and treated with different concentrations of NS-398. The expressions of COX-2 and Survivin mRNA were detected by RT-PCR method and the expressions of COX-2 and Survivin protein were detected by flow eytometry/finaneal capacity model（FCM） and Western blot method. Results： There was a high expression of COX-2 and Survivin gene/protein in SMMC-7721 cells. NS-398 inhibited the expression of COX-2 and Survivin expression in SMMC-7721 cells in the manner of dose and time dependent. Conclusion： NS-398 could inhibit proliferation and induce apoptosis of SMMC-7721 by inhibiting the expression of COX-2 and Survivin.