多基因遗传表达分析系统检测rhIL-24诱导人卵巢癌细胞凋亡相关基因的应用

The detection of apoptosis-associated genes in SKOV3,SKOV3 /ddp cells of the recombinant human interleukin-24 induced by GeXP analysis system

目的:探讨重组人白细胞介素24(recombinant human interleukin-24,rhIL-24)诱导人卵巢癌SKOV3细胞株、SKOV3/ddp人卵巢癌耐顺铂细胞株的凋亡相关6个基因表达的变化。方法:体外培养人卵巢癌SKOV3细胞株、人卵巢癌耐顺铂SKOV3/ddp细胞株,用rhIL-24单独及其联合顺铂作用于两种不同的细胞株,提取RNA,经逆转录和聚合酶链反应,应用多基因遗传表达分析系统(GeXP)同时检测6个凋亡基因。结果:rhIL-24诱导上调人卵巢癌SKOV3细胞株Bax、Rb、P53基因,激活Caspase-3,下调Bcl-2、Birc5基因;rhIL-24诱导上调人卵巢癌耐顺铂SKOV3/ddp细胞株Bax、Rb基因,激活Caspase-3,下调Bcl-2基因;rhIL-24联合DDP组其上述基因表达变化更加显著。结论:rhIL-24诱导人卵巢癌细胞凋亡主要通过上调Bax、Rb、P53,激活Caspase3,下调Bcl-2,Birc5等凋亡相关基因。

Objective：To detect the change of apoptosis-associated genes in human ovarian cancer cell lines SKOV3 cells and SKOV3/ddp cells, which were induced by the recombinant human interleukin-24 （rhlL-24）. Methods： The human ovarian cancer cell lines SKOV3 cells and SKOV3/ddp cells in vitro culture, both of the cells were induced by rhlL-24, and rhlL-24 combined with eispla- tin （DDP）. Extracting RNA, and by revesing transcription and polymerizing chain reaction to detect six genes expression level by GeXP genetic analysis system at the same time. Results： The genes expression level of Caspase-3, Bax, Rb, P53 was obviously unreg- ulated; and Bcl-2, Birc5 was down regulated in SKOV3 cells after induced by rhlL-24. On the SKOV3/ddp cells was unregulated the Caspase-3, Bax, Rb. And down regulate the Bcl-2. All the results were more obviously when all genes mentioned above induced by rhlL-24 combined with DDP. Conclusion： The potential mechanism of rhlL-24 on human ovarian cancer cells in vitro relate to the ap- optosis-associated genes expression level through the up regulation of Caspase-3, Bax, Rb, P53 and down regulation of Bcl-2, Birc5.