马兜铃水提液对斑马鱼胚胎的致畸作用和心脏毒性的研究

Study on Teratogenesis and Cardiac Toxicity of Aqueous Extraction of Aristolochia contorta to Zebrafish Embryos

目的:探索马兜铃水提液对斑马鱼胚胎的致畸作用和心脏毒性。方法:分别用不同浓度的马兜铃水提液和马兜铃酸A(AA)处理斑马鱼胚胎,观察致畸作用和对心脏发育影响。结果:给药组的斑马鱼胚胎出现畸形和死亡;当水提液中AA含量为0.5μg/mL时,胚胎心率明显减慢;AA含量为5μg/mL时,胚胎在24~48 hpf之间全部死亡;水提液的LC50为1.43μg/mL。结论:与AA相比,马兜铃水提液对斑马鱼胚胎有着更强的致畸和心脏毒性,且毒性作用具有时间和浓度依赖性。

Object： To explore the developmental toxicity of aqueous extraction of Aristolochia contorta to zebrafish embryos. Methods： Aristolochic acid A （AA） was first tested on zebrafish embryo model to ob- serve the teratogenesis and cardiac effect. Then aqueous extraction of Aristolochia contorta was investiga- ted on wild type zebrafish embryos, and the zebrafish embryos were observed under microscope to see the teratogenesis and cardiac effect. Results： After soaking zebrafish embryos in aqueous extraction for 42 h, the embryos displayed malformed phenotypes. Besides the concentration of AA reaching 0.5 p.g/mL, the heart beats reduced obviously compared to the control group. When the concentration of AA was more than 5 p^g/mL, none of the embryos survived after 48 hpf. The heart rates of all the tested groups of Aris- tolochia contorta were lower than that of the control group. The LCs0 of Aristolochia contorta was 1. 43 μg/ mL. Conclusions： Aristolochia contorta displayed stronger toxicity to embryos including teratogenesis, cardiac toxicity and developmental inhibition than AA.