藏药毛蕊杜鹃挥发油化学成分的GC-MS分析

GC- MS Analysis of the Chemical components of Essential Oil in Rhododendron websterianum

目的:通过研究毛蕊杜鹃挥发油的化学成分,为毛蕊杜鹃的药用及开发利用提供依据。方法:采用水蒸气蒸馏法提取毛蕊杜鹃挥发油,用GC毛细管柱进行分离,归一化法测其相对含量,并用气相色谱-质谱法对化学成分进行鉴定。结果:共鉴定出38个化合物,鉴定出的化合物含量占总挥发油的92.96%。结论:毛蕊杜鹃挥发油主要成分为δ-杜松烯(相对含量为15.90%);胡萝卜醇(15.09%);α-杜松醇(15.02%);τ-杜松醇(9.66%);α-衣兰油烯(4.25%);1,2,3,4,4a,5,6,8a-八氢-7-甲基-4-亚甲基-1-[1-甲基乙基]-萘(4.14%);1,7-二甲基-4-异丙基-2,7-环癸二烯-1-羟基(3.27%);石竹烯(2.86%);5-甲基-9-亚甲基-2-异丙基-二环[4.4.0]癸-1-烯(2.62%);1,4-二甲基-3-[2-甲基-1-丙烯-1-基]-4-乙烯基-1-环庚烯(2.42%);γ-衣兰油烯(2.21%);愈创醇(1.50%);β-古芸烯(1.34%)。

Objective ： In this paper, the chemical constituents of volatile oil from Rhododendron webste- rianum was studied. The research aimed to provide scientific basis for exploitation of R. websterianum. Methods： The volatile oil was extracted by steam distillation, and then analyzed by GC-MS. Results： 38 compounds in the volatile oil were identified, and those compounds account for 92.96% of the total vola- tile oil. Conclusions ： The major components of the volatile oil is Daha-cadinene （ 15.90% ）, Carotol （15.09%）, alpha-Cadinol （15.02%）, tau-Cadinol （9.66%） , alpha-Muurolene （4.25 % ）, Naphtha- lene, 1,2, 3,4, 4a, 5, 6, 8a-octhydro-7-methyl-4-methylene-l-[ 1-methylethyl]-, [ 1 alpha, 4a beta, 8a. alpha ]-（4. 14% ）, 1-Hydrooxy-1, 7-dimethyl-4-isopropyl-2, 7-cyclodeeadiene （3.27%）, Caryo- phyllene （2.86%）, Bieyclo [ 4.4.0 ] dec-1 -ene, 2-isopropyl-5-methyl-9-methylene- （ 2.62% ）, 1 -Cyelo- heptene, 1,4-dimethyl-3- [ 2-methyl-1 -propene-1 -yl ] -4-vinyl-（ 2.42 % ）, gamma-Muurolene （ 2.21% ）, Guaiol （ 1.50）, beta-Gurjunene（ 1.34% ）.