紫外线诱导巨型艾美耳球虫的早熟株选育

SELECTION OF A PRECOCIOUS STRAIN OF EIMERIA MAXIMA THROUGH ULTRAVIOLET RADIATION

本试验分离获得了1株巨型艾美耳球虫,通过紫外线诱导和非紫外线诱导两种方法对该虫株进行早熟选育,获得了2个巨型艾美耳球虫早熟减毒株。通过对2个早熟株的潜隐期、繁殖力、致病力以及免疫原性进行对比研究,证明紫外线诱导下的早熟选育可明显缩短选育所需代数,且与传统方法选育的早熟株在繁殖力、免疫原性与免疫保护力等方面基本相同。紫外线诱导下,经7代早熟选育,潜隐期由母株的142 h缩短到116 h,而非紫外线诱导的选育需要20代才能达到同样的选育结果。两早熟株卵囊的繁殖能力与母株相比有所下降,但仍保留了母株原有的免疫原性。

Two precocious strains were selected from an Eimeria maxima strain that was isolated from chicken feces. One precocious strain was treated with ultraviolet radiation while other strain was not. Then these two precocious strains were compared for their prepatent periods, reproductive potentials, pathogenicity and immunogenicity. The prepatent period of the treated precocious strain was shortened from 142 h to 116 h within 7 passages. In the traditional passages without ultraviolet radiation, however, 20 passages were required to reduce the prepatent time from 142 h to 117 h. The reproductive potential and immunogenicity were found no difference in these two precocious strains. The present study demonstarted that ultraviolet treatment of Eimeria maxima significantly reduced passage time to obtain precocious strains.