摘要
目的探讨雌激素受体(ER)、孕激素受体(PR)及基质金属蛋白酶-2(MMP-2)在子宫腺肌病(UAM)中的表达和意义。方法应用免疫组化二步法检测35例UAM患者的异位内膜、在位内膜及38例非UAM患者正常内膜中ER、PR和MMP-2的表达情况,通过统计学方法行对照研究。结果 (1)UAM异位内膜组织中ER、PR的表达较对照正常内膜低(P<0.05);(2)UAM异位内膜中MMP-2表达水平显著高于正常内膜组(P<0.01);(3)MMP-2蛋白在UAM异位内膜中表达有周期性变化,增生期高于分泌期(P<0.05),而在在位内膜及对照内膜中表达无明显周期性变化。结论 ER表达并非子宫腺肌病的主要致病因素;MMP-2在异位内膜的高表达可能使异位内膜具有更高的侵袭性,并且在增生期表达活跃。
Objective To explore the expression and significance of ER, PR and MMP - 2 in Uterine Adenomy- osis (UAM). Methods Immunohistochemical method was employed to detect the expression of estrogen(ER) ,pro- gesterone receptor(PR) and Matrix Mealloproteinase- 2( MMP-2) in the ectopic endometrium and the eutopic en- dometrium of 35 patients with UAM and the normal controls( n = 38 ) ,then the correlation of the expression of them was analyzed. Results ( 1 ) The expression of ER and PR in ectopic endometrium were significantly lower than that in normal endometrium ( P 〈 O. 05 ) ; (2) The expression of MMP - 2 was obviously higher in the ectopic endometri- um of UAM patients than that of controls( P 〈0. O1 ) ;(3 )The expression of MMP-2 protein in ectopic endometri- um showed cyclic change obviously, the expression level of MMP -2 in proliferate stage was significantly higher than that in protestation stage (P 〈 0.05 ). But in eutopic endometrium and the normal controls showed no cyclic change. Conclusions The high expression of MMP - 2 in the ectopic endometrium suggests that the ectopic endome- trium with UAM may be more invasive and the expression was higher in proliferation stage.
出处
《青海医学院学报》
CAS
2013年第4期274-277,共4页
Journal of Qinghai Medical College