正常和失眠症患者血清神经肽Y和P物质对照研究

A CONTROLED STUDY OF SERUM LEVELS OF NEUROPEPTIDE Y AND SUBSTANCE P IN PATIENTS WITH INSOMNIA AND NORMAL CONTROLS

目的:探讨正常和失眠症患者血清神经肽Y(NPY)和P物质(SP)的特征。方法:选取国际疾病分类诊断标准(ICD-10)之相应诊断标准的失眠症患者75例及64名正常成人,以酶联免疫吸附法测定血清NPY及SP水平,以汉密尔顿焦虑量表(HAMA)及匹茨堡睡眠质量指数量表(PSQI)评定焦虑症状和主观睡眠质量状况。结果:(1)失眠症组血清NPY水平低于正常对照组(P<0.01)。(2)血清SP水平高于正常对照组(P<0.05)。(3)血清SP水平与病程、HAMA总分和PSQI总分无明显相关。结论:失眠症患者可能存在外周NPY及SP能神经元功能紊乱,值得进一步随访。

Objective：To investigate the characteristic of the serum levels of neuropeptide Y（NPY）and substance P（SP）between patients with insomnia and normal controls. Methods：75 patients with insomnia who met the criteria of ICD-10（The International Statistical Classification of Diseases and Related Health Problems 10th Revision,ICD-10）,and 64 normal controls were enrolled in the study. The serum NPY and SP concentrations were determined by enzyme-linked immunosorbent assay. The Hamilton Anxiety Scale（HAMA）,and Pittsburgh Sleep Quality Index（PSQI）were used to assess the subject＇s anxiety symptoms and subjective sleep quality. Results：① Significant decreased serum NPY levels were found in patients with insomnia compared with the control group（P0.01）. ② Patients with insomnia showed significant higher serum SP levels compared with the control group（P0.05）. ③ No significant correlation were found between SP levels and course of disease,total scores of HAMA and PSQI. Conclusion：Patients with insomnia may have dysfunction of peripheral SP and NPY neuron,which deserves further study.