疱疹病毒片的抗炎抑菌作用研究

Studies on Anti-inflammatory and Antibacterial Activities of Paozhen Bingdu Tablet

目的研究疱疹病毒片的抗炎、抑菌作用。方法将小鼠、大鼠随机分为模型组、阳性药物组和疱疹病毒片高、中、低剂量组(按生药计分别为10.2,6.8,3.4 g·kg-1),通过小鼠耳肿胀、小鼠腹腔毛细血管渗出实验、小鼠棉球肉芽肿及大鼠足趾肿胀模型观察其抗炎作用;通过体外、体内抑菌实验,观察其抑菌作用。结果与模型组比较,疱疹病毒片高剂量能减轻小鼠耳廓肿胀度,抑制小鼠腹腔毛细血管渗出,降低大鼠足趾肿胀程度,减轻棉球肉芽肿质量,差异有统计学意义(P<0.05);疱疹病毒片对金黄色葡萄球菌、表皮葡萄球菌、志贺痢疾杆菌、不动杆菌、肠球菌、铜绿假单胞菌、大肠埃希菌、福氏痢疾杆菌、肺炎克雷白杆菌、阴沟杆菌、白念珠菌、乙型溶血性链球菌、肺炎链球菌均有抑制作用,其最小抑菌浓度分别为1.00,1.00,4.00,4.00,4.00,4.00,4.00,4.00,4.00,4.00,4.00,4.00,4.00 g·mL-1(按生药计),并可以降低金黄色葡萄球菌和表皮葡萄球菌感染小鼠的死亡率。结论疱疹病毒片具有抗炎、抑菌作用。

Objective To study the anti-inflammatory and antibacterial effect of paozhen bingdu tablet. Methods Mice were divided into 5 groups randomly：normal saline group, positive group, high, middle and low dose groups ofpaozhen bingdu tablet（ 10.2, 6.8, 3.4 g · kg-1, respectively）. The anti-inflammatory effect of paozhen bingdu tablet was observed via mouse earswelling model and cotton-pellet-induced granuloma and paw edema model, mad by using mouse peritoneal capillary oozing test. The antibacterial effect ofpaozhen bingdu tablet was observed through in vitro and in vivo antibacterial test. Results The high dose of paozhen bingdu tablet significantly relieved ear-swelling, inhibited peritoneal capillary oozing, and reduced paw edema degree and cotton granuloma weight （ P〈0.05 ）. Paozhen bingdu tablet had in vitro antibacterial effects on Staphylococcus aureus, Staphylococcus epidermidis, Shigella dysentery bacillus, Acinetobacter, Enterococcus , Pseudomonas aeruginosa, E. coli, ShigeUa flexneri, Klebsiella pneumoniae, Enterobacter cloacae, Candida albicaus, B Streptococcus, and Pulmonary Streptococcus. The minimum inhibitory concentration（MIC） was 1.00, 1.00, 4.00, 4.00, 4.00, 4.00, 4.00, 4.00, 4.00, 4.00, 4.00, 4.00, 4.00 g· mL-1, respectively. Paozhen bingdu tablet reduced mortality in mice infected with Staphylococcus aureus and Staphylococcus epidermidis. Conclusion Paozhen bingdu tablet has anti-inflammatory and antibacterial effects.