脑苷肌肽注射液对PC-12及SH-SY5Y细胞的保护作用

Protective Effect of Encephalon Glycoside and Ignotin Injection on PC-12 and SH-SY5Y Cells

目的 比较不同浓度脑苷肌肽注射液对体外培养的大鼠嗜铬细胞瘤PC-12细胞及人神经母细胞瘤SH-SY5Y细胞的保护作用.方法 通过噻唑蓝法（MTT）检测细胞存活率;应用ELISA法检测细胞培养液中神经元特异性烯醇化酶（NSE）和脑源性神经营养因子（BDNF）水平.结果 脑苷肌肽注射液在0.1～300.0 μg·mL-1（以多肽计）浓度范围内对0.000 34%过氧化氢（H2O2）诱导PC-12损伤有保护作用,最大效应（43.0±9.2）%,在0.1～30.0 μg·mL-1（以多肽计）浓度范围内对H2O2诱导的SH-SY5Y细胞损伤有保护作用,最大效应为（50.5±11.9）%.ELISA法检测发现,脑苷肌肽注射液可使两损伤细胞株培养液中NSE水平显著减少,且以SH-SY5Y更为显著;BDNF水平在两细胞株中均显著增加.结论 脑苷肌肽注射液对H2O2诱导的PC-12及SH-SY5Y细胞的损伤均有保护作用,且以人源性SH-SY5Y细胞更为敏感;该过程伴有NSE水平降低,脑苷肌肽细胞保护作用机制可能与增加BDNF表达有关.

Objective To determine and compare the protective effect of different concentrations of encephalon glycoside and ignotin injection on rat pheochromocytoma PC-12 cells and human neuroblastoma SH-SYSY tumor cells. Methods The cell survival rates and the levels of NSE and BDNF in cellular media were detected by thiazole blue method（MTT） and ELISA respectively. Results Encephalon glycoside and ignotin injection within the polypeptide concentration range of 0.1 -300.0 μg · mL-1 exerted protective effect on PC-12 cell injury induced by 0.000 34% H202, and the highest protective rate was （43.0±9.2）%. In the polypeptide concentration range of 0. 1-30.0 μg· mL-1, encephalon glycoside and ignotin injection effectively protected SH-SY5Y cell injury induced by 0. 0003 34% H2O2 , with the highest protective rate being（50.5±11.9） %. In ELISA, NSE in both SH-SY5Y and PC-12 cell culture media was significantly decreased by encephalon glycoside and ignotin injection, while BDNF was significantly increased. Conclusion Encephalon glycoside and ignotin injection has a protective effect on H2O2-induced PC-12 and SH-SY5Y cell injury. NSE is down-regulated during the treatment process. The upregulation of BDNF may be involved in the mechanism of protection of encephalon glycoside and ignotin injection on the cells.