丹参不同组分对非酒精性脂肪肝大鼠肝脏PPARαmRNA表达的影响

Effect of Different Components of Danshen on PPARαmRNA Expression in Rats with Non-alcoholic Fatty Liver

目的:观察中药丹参的不同组分(丹参总酮和总酚酸)对实验性非酒精性脂肪肝(NAFLD)的治疗效果和作用机制。方法:通过喂食高脂饲料复制NAFLD大鼠模型,将大鼠分为正常对照组、模型对照组、东宝肝泰对照组、丹参总酮组和丹参总酚酸组。用药8周后,检测各组大鼠血清中TC、TG、ALT、AST和肝组织中TC、TG的含量或活性变化,用RT-PCR法观察各组大鼠肝脏PPARαmRNA的表达变化,并用HE染色法观察肝组织的形态学变化。结果:丹参两种组分都能降低实验动物血清中TC、TG、ALT、AST的含量或活性,降低和肝组织中TC、TG的含量,促进肝脏PPARαmRNA的表达,改善肝组织病理形态学。结论:丹参总酮和总酚酸防治NAFLD的作用机制可能与增强肝组织中PPARαmRNA的表达,进而促进脂肪酸代谢有关。

Objective：to observe the effect and mechanism of different components of Danshen （ total ketone and total phenolic acid） on non-alcohol fatty liver （ NAFLD） .Methods： Feeding high-fat forage to reproduce NAFLD model rats , divide the rats into control group, model group, Dongbao Gantai group, ketone group and phenolic acid group.8 weeks after administration , detect TC, TG, ALT, AST in blood serum, and TC, TG contents and activity in hepatic tissue;observe the PPARαmRNA expressions with RT-PCR and observe the morphologic variations of hepatic tissue with HE method.Results：both components of Danshen could decrease the con-tents and activities of TC, TG, ATL and AST in serum, decrease TC and TG in hepatic tissue, enhance the PPARαmRNA expression, and improve the pathomorphism of hepatic tissue.Conclusion：The mechanism of Danshen total ketone and total phenolic acid in preven-ting and curing NAFLD may be related to their enhancing PPARαmRNA expression so to improve the metabolism of fatty acids.