场增强样品堆积-毛细管电泳法测定山楂粉中的氨基酸

Determination of Amino Acids in Crataegus Pinnatifida by Capillary Electrophoresis Coupled with Field Enhanced Sample Stacking

采用场增强样品堆积-毛细管电泳法建立了在线富集氨基酸的分析方法，用于中药山楂中水解氨基酸的检测，并进行了回收率实验．采用富集电压为-20kV，进样压力为3psi，进样时间为50S，紫外检测波长为214nm，运行缓冲溶液为270mmol／L乙酸-270mmol／L乙酸钠（pH=4．15）-6％（V／V）乙腈溶液，分离电压为17kV，组氨酸、精氨酸、色氨酸、酪氨酸、缬氨酸、苯丙氨酸、亮氨酸、苏氨酸、丙氨酸、甘氨酸、谷氨酸和门冬氨酸等12种氨基酸在50min内达到分离，检出限在0．0003～0．08μgmL之间．

A method for the amino acid analysis by capillary electrophoresis coupled with field enhanced sample stacking has been developed. This method has been applied to determine the content of hydrolyzed amino acids in Crataegus pinnatifida and the recovery experiment was also carried out. The optimum enrichment and separation conditions are as follows： enrichment voltage is -20 kV, injection time is 50 s （at 3 psi） , UV detection wavelength is 214 nm, buffer solution is 270 mmoL/L acetic acid-270 mmol/L sodium acetate （pH = 4.15 ）-6% （V/V） acetonitrile, the separation voltage is 17 kV. Twelve amino acids （histidine, arginine, tryptophane, tyrosine, valine, phenylalanine, leucine, threonine, alanine, glycine, glutamic acid and aspartic ） were separated and determined simultaneously in 50 minutes under the above optimum conditions. The detection limits （S/N = 3 ） ranged from 0.000 3 to 0.08 μg/mL.