HPLC-AFS法分析海藻类产品中的As(Ⅲ)和As(Ⅴ)

Analysis of As(Ⅲ) and As(Ⅴ) in Seaweed Food by HPLC-AFS

建立了分析海藻类产品中2种无机砷价态的高效液相色谱-原子荧光光谱（HPLC—AFS）联用的分析方法．样品经稀硝酸热浸提后离心，取上清液过C18小柱及0．22μm滤膜，进样分析．结果表明：2种无机砷在5．0～100．0μg/L范围内呈良好的线性关系，相关系数r均大于0．999，As（Ⅲ）的最低定量限为0．01mg／kg，As（V）的最低定量限为0．02mg／kg，As（Ⅲ）和As（V）的样品加标回收率为86．2％～106．5％，相对标准偏差（RSD）为3．47％-6．14％．方法可满足海藻类产品中2种价态无机砷的含量分析要求．

An analytical method for the determination of As（III） and As（V） in seaweed products is established using HPLC-AFS. The samples were extracted with dilute nitric acid and then centrifuged. After a solid-phase extraction, the analytes were quantified on a Hamilton PRP X-100 column （250×4.1 mm, 10 μm）with gradient elution. The linear correlation coefficients of As （III） and As （V） were greater than 0. 9990. The average recoveries of the two inorganic arsenic species ranged from 90.3% - 106.5%. The method is effective and sensitive, and is suitable for the analysis of inorganic arsenic speciation in seaweed products.