摘要
建立了分析海藻类产品中2种无机砷价态的高效液相色谱-原子荧光光谱(HPLC—AFS)联用的分析方法.样品经稀硝酸热浸提后离心,取上清液过C18小柱及0.22μm滤膜,进样分析.结果表明:2种无机砷在5.0~100.0μg/L范围内呈良好的线性关系,相关系数r均大于0.999,As(Ⅲ)的最低定量限为0.01mg/kg,As(V)的最低定量限为0.02mg/kg,As(Ⅲ)和As(V)的样品加标回收率为86.2%~106.5%,相对标准偏差(RSD)为3.47%-6.14%.方法可满足海藻类产品中2种价态无机砷的含量分析要求.
An analytical method for the determination of As(III) and As(V) in seaweed products is established using HPLC-AFS. The samples were extracted with dilute nitric acid and then centrifuged. After a solid-phase extraction, the analytes were quantified on a Hamilton PRP X-100 column (250×4.1 mm, 10 μm)with gradient elution. The linear correlation coefficients of As (III) and As (V) were greater than 0. 9990. The average recoveries of the two inorganic arsenic species ranged from 90.3% - 106.5%. The method is effective and sensitive, and is suitable for the analysis of inorganic arsenic speciation in seaweed products.
出处
《分析测试技术与仪器》
CAS
2013年第4期217-221,共5页
Analysis and Testing Technology and Instruments